IDH1/2 Mutation Detection Kit

Cat Number: IDH-RT38

The IDH1/2 Mutation Detection Kit is a PCR-based test that identifies mutations in the IDH1 and IDH2 genes, key biomarkers in diffuse gliomas and acute myeloid leukemia (AML).

38 tests per kit
Less than 2-hour turnaround time
Works with FFPE and fresh frozen samples
5 Reactions per sample
For research use (RUO) in the U.S
For in vitro diagnostic use (IVD) in the European Union

Categories: Glioblastoma, Leukemia Tags: IDH1, IDH2

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Mutations in the IDH1 and IDH2 genes are among the most significant genetic alterations in diffuse gliomas and serve as key biomarkers for tumor classification, prognosis, and treatment planning. Studies have shown that IDH1/2 mutations are highly prevalent in lower-grade gliomas (WHO grades II and III) and secondary glioblastomas, where they are associated with better patient survival outcomes. Additionally, these mutations are known to influence tumor metabolism, making them potential targets for emerging therapeutic strategies.

In addition to their role in gliomas, IDH1/2 mutations are found in approximately 20% of acute myeloid leukemia (AML) patients. These mutations disrupt key cellular processes, preventing bone marrow cells from maturing properly and contributing to leukemia development. Targeting these mutations, IDH inhibitors have been developed to help leukemia cells differentiate into normal blood cells. Several of these targeted therapies have been approved for treating AML patients with specific IDH1/2 mutations, highlighting the importance of comprehensive mutation testing for informing therapeutic strategies.

The IDH1/2 Mutation Detection Kit is a real-time PCR-based assay for accurate and sensitive mutation detection. This assay is designed to preferentially amplify mutant DNA, ensuring reliable results even in samples with a high proportion of wild-type DNA.

The test provides a rapid and robust solution for identifying IDH1 and IDH2 mutations in FFPE and fresh/frozen tissue samples.

Gene	Exon	aa change	nt change	Cosmic ID	Detected by Primer No.
IDH1	4	R132H	c.395G>A	COSM28746	1
		R132C	c.394C>T	COSM28747
		R132S	c.394C>A	COSM28748
		R132G	c.394C>G	COSM28749
		R132L	c.395G>T	COSM28750
		R132P	c.395G>C	COSM221574
		R132V	c.394_395CG>GT	COSM28751
		R100Q	c.299G>A	COSM88208	2
IDH2	4	R172K	c.515G>A	COSM33733	3
		R172M	c.515G>T	COSM33732
		R172W	c.514A>T	COSM34039
		R172G	c.514A>G	COSM33731
		R172S	c.516G>T	COSM34090
		R140Q	c.419G>A	COSM41590	4
		R140W	c.418C>T	COSM41877	4
		R140L	c.419G>T	COSM41875	5
		R140G	c.418C>G	COSM1737874	5

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The IDH1/2 Mutation Detection Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.

EntroGenʼs IDH1/2 mutation detection kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. This test includes reagents required for PCR amplification/detection, as well as validated reaction controls.

Columns and reagents for DNA isolation are not included. Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA® Genomic DNA Isolation Kit.