NRAS Mutation Analysis Kit
Cat Number: NRAS-RT50
The NRAS Mutation Analysis Kit is a real-time PCR-based assay that detects somatic mutations in the NRAS gene, which are associated with tumor progression and resistance to EGFR-targeted therapies.
- Below 1% Limit of Detection
- 50 tests per kit
- 2-hour turnaround time
- Works with FFPE and fresh frozen samples
- 10 Reactions per sample
- For research use (RUO) in the U.S
- For in vitro diagnostic use (IVD) in the European Union
Mutations in the NRAS gene, a key member of the RAS family of small GTPases, play a crucial role in tumor development by driving uncontrolled cell proliferation through the RAF-MEK-ERK pathway and enhancing cell survival via the PI3K-AKT-mTOR pathway. Studies have shown that tumors harboring NRAS mutations exhibit reduced responsiveness to EGFR-targeted therapies, making NRAS mutation analysis essential for guiding treatment decisions.
The NRAS Mutation Analysis Kit is a real-time PCR-based assay designed to detect clinically relevant somatic mutations in the NRAS gene, even in samples containing a mixture of wild-type and mutant DNA.
Using mutation-specific primers and fluorescent hydrolysis probes, the assay ensures highly specific amplification of mutant variants while also incorporating an internal control gene to verify sample quality.
This robust and sensitive assay is optimized for use with FFPE and fresh/frozen tissue samples, providing reliable results to support precision oncology and therapeutic decision-making.
The table below lists the mutations detected by the NRAS Mutation Detection kit.
| Exon | Mutation Name | Nucleotide Change | Amino Acid Change |
| 2 | G12C | c.34G>T | Glycine (G) to Cysteine (C) |
| G12D | c.35G>A | Glycine (G) to Aspartic acid (D) | |
| G12S | c.34G>A | Glycine (G) to Serine (S) | |
| G13V | c.38G>T | Glycine (G) to Valine (V) | |
| G13R | c.37G>C | Glycine (G) to Arginine (R) | |
| 3 | Q61K | c.181C>A | Glutamine (Q) to Lysine (K) |
| Q61R | c.182A>G | Glutamine (Q) to Arginine (R) | |
| Q61L | c.182A>T | Glutamine (Q) to Leucine (L) | |
| Q61H | c.183A>C & c.183A>T | Glutamine (Q) to Histidine (H) | |
| 4 | A146T | c.436G>A | Alanine (A) to Threonine (T) |
The NRAS Mutation Analysis Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.
EntroGenʼs NRAS mutation screening panel requires a real-time PCR instrument capable of detecting VIC and FAM fluorescent probes.
This test includes reagents required for PCR amplification/detection, as well as validated reaction controls.
Columns and reagents for DNA isolation are not included. Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA® Genomic DNA Isolation Kit.
We suggest using the Internal Quality Control Assay (cat. no. IQCA–RT50) to determine the optimal amount of input DNA prior to running this assay.
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