PIK3CA Mutation Analysis Kit for Real-Time PCR
Cat Number: PI3K-RT48
The PIK3CA Mutation Analysis Kit is a PCR-based assay that detects somatic mutations in the PIK3CA gene, which are associated with cancer growth and therapeutic resistance, particularly in HR-positive, HER2-negative breast cancer and KRAS wild-type metastatic colorectal cancer.
- 48 tests per kit
- 2-hour turnaround time
- Works with FFPE and fresh frozen samples
- 6 Reactions per sample
- For research use (RUO) in the U.S
- For in vitro diagnostic use (IVD) in the European Union
Mutations in the PIK3CA gene are frequently found in various cancers, including HR-positive, HER2-negative breast cancer and KRAS wild-type metastatic colorectal cancer. These mutations contribute to cancer progression and resistance to certain therapies. Studies indicate that patients with PIK3CA mutations may benefit from PI3Kα-specific inhibitors, making genetic screening a crucial step in treatment planning.
The PIK3CA Mutation Analysis Kit uses real-time PCR to accurately detect somatic mutations in the PIK3CA gene, even in samples containing a mixture of mutant and wild-type DNA. The assay includes an internal control to ensure reliable results and is designed to support therapeutic decision-making for patients with PIK3CA-mutated cancers.
The PIK3CA Mutation Analysis Kit is a qPCR assay designed to detect 11 different somatic mutations in a total of 6 reactions per sample.
| Reaction | Mutation | Reporter |
| 1 | E542K | ROX |
| H1047Y | FAM | |
| 2 | E545K | ROX |
| C420R | FAM | |
| 3 | E545G | ROX |
| E545D | CY5 | |
| 4 | E545A | ROX |
| H1047L | FAM | |
| 5 | Q546E | ROX |
| H1047R | FAM | |
| 6 | Q546R | FAM |
The PIK3CA Mutation Analysis Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.
EntroGenʼs PIK3CA mutation screening panel requires a real-time PCR instrument capable of detecting FAM, ROX, and CY5 fluorescent probes.
This test includes reagents required for PCR amplification/detection, as well as validated reaction controls.
Columns and reagents for DNA isolation are not included. Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA® Genomic DNA Isolation Kit.
