PIK3CA Mutation Analysis Kit for Real-Time PCR

Cat Number: PI3K-RT48

The PIK3CA Mutation Analysis Kit is a PCR-based assay that detects somatic mutations in the PIK3CA gene, which are associated with cancer growth and therapeutic resistance, particularly in HR-positive, HER2-negative breast cancer and KRAS wild-type metastatic colorectal cancer.

 

  • 48 Reactions per kit
  • 2-hour turnaround time
  • Works with FFPE and fresh frozen samples
  • 6 Reactions per sample
  • For research use (RUO) in the U.S
  • For in vitro diagnostic use (IVD) in the European Union

Mutations in the PIK3CA gene are frequently found in various cancers, including HR-positive, HER2-negative breast cancer and KRAS wild-type metastatic colorectal cancer. These mutations contribute to cancer progression and resistance to certain therapies. Studies indicate that patients with PIK3CA mutations may benefit from PI3Kα-specific inhibitors, making genetic screening a crucial step in treatment planning.

The PIK3CA Mutation Analysis Kit uses real-time PCR to accurately detect somatic mutations in the PIK3CA gene, even in samples containing a mixture of mutant and wild-type DNA. The assay includes an internal control to ensure reliable results and is designed to support therapeutic decision-making for patients with PIK3CA-mutated cancers.

The PIK3CA Mutation Analysis Kit is a qPCR assay designed to detect 11 different somatic mutations in a total of 6 reactions per sample.

 

Reaction Mutation Reporter
1 E542K ROX
H1047Y FAM
2 E545K ROX
C420R FAM
3 E545G ROX
E545D CY5
4 E545A ROX
H1047L FAM
5 Q546E ROX
H1047R FAM
6 Q546R FAM

The PIK3CA Mutation Analysis Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a far less complicated process.

EntroGenʼs PIK3CA mutation screening panel requires a real-time PCR instrument capable of detecting FAM, ROX, and CY5 fluorescent probes.

This test includes reagents required for PCR amplification/detection, as well as validated reaction controls.
Columns and reagents for DNA isolation are not included.

 

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