MLL-AF4 One-Step Detection Kit
Cat Number: LEUK3-QRT24
The MLL-AF4 One-Step Detection Kit is a highly sensitive and specific molecular diagnostic tool designed for the detection and quantification of MLL-AF4 fusion transcripts optimized for both screening and monitoring residual or recurrent childhood and adult precursor-B-ALL.
- Analytical sensitivity of 10-5
- One –step cDNA synthesis
- 24 tests per kit
- Less than 2-hour turnaround time
- Works with peripheral blood or bone marrow
- For research use (RUO) in the U.S
- For in vitro diagnostic use (IVD) in the European Union
Chromosomal translocations are key genetic events in leukemia, leading to the formation of fusion genes that drive disease progression. One of the most significant translocations in childhood and adult precursor-B-ALL is t(4;11)(q21;q23), resulting in the MLL-AF4 fusion gene, which is present in approximately 5% of childhood and adult precursor-B-ALL cases. This fusion gene plays a crucial role in leukemogenesis by disrupting normal hematopoietic differentiation.
The presence of these fusion genes is a critical factor in leukemia diagnosis and monitoring, enabling clinicians to assess disease burden, detect minimal residual disease (MRD), and identify early molecular relapse for timely therapeutic intervention.
The MLL-AF4 One-Step Detection Kit is a highly sensitive and specific real-time PCR-based assay designed to detect and quantify multiple leukemia-associated fusion transcripts in RNA extracted from blood or bone marrow samples. The assay utilizes a FAM-labeled hydrolysis probe for detecting the target fusion transcripts, along with a Cal Fluor Orange 560 (VIC equivalent)-labeled probe targeting the ABL gene as an internal control. This dual-target design enables accurate quantification using the comparative Ct method (Pfaffl method), eliminating the need for external reference standards.
The use of optimized primers and reagents ensures efficient and reproducible cDNA synthesis in a single-tube reaction, enhancing sensitivity and specificity.
The MLL-AF4 One-Step Detection Kit follows a simple and easy to use process. It can be completed in 2 hours.
EntroGenʼs MLL-AF4 One-Step Detection Kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. This test includes reagents required for PCR amplification/detection, as well as validated reaction controls.
The MLL-AF4 One-Step Detection Kit features a streamlined workflow. It includes first-strand cDNA synthesis and subsequent real-time PCR in a single tube.
The kit does not include reagents or columns for RNA isolation.
