JAK2 V617F Mutation Analysis Kit
Cat Number: MUT-RT50
The MUT-RT50 JAK2 V617F Mutation Analysis Kit is a highly sensitive and specific molecular diagnostic tool designed for the detection and quantification of the JAK2 V617F mutation, optimized for assessing and guiding therapeutic decisions in various malignancies.
- 50 tests per kit
- Detects low copy JAK2 V617F mutation in genomic DNA
- Less than 2-hour turnaround time
- Works with peripheral blood or bone marrow
- For research use (RUO) in the U.S and the European Union
The JAK2 V617F mutation results in a constitutively active JAK2 kinase, driving uncontrolled cellular proliferation and survival. This mutation arises from a valine (V) to phenylalanine (F) substitution at codon 617, causing JAK2 activation independent of upstream signals. The JAK2 V617F mutation has been identified in various malignancies and is associated with reduced sensitivity to JAK2 inhibitors, distinguishing it from other oncogenic driver mutations such as EGFR, KRAS, and ALK mutations.
The MUT-RT50 JAK2 V617F Mutation Analysis Kit is a highly sensitive and specific real-time PCR-based assay designed to detect the JAK2 V617F mutation in DNA extracted from blood or bone marrow samples. The assay employs mutation-specific primers that preferentially amplify mutant DNA, ensuring highly specific detection even in a background of wild-type DNA.
The JAK2 V617F Mutation Analysis Kit follows a simple and easy to use process. It can be completed in 2 hours.
EntroGenʼs JAK2 V617F Mutation Analysis Kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. This test includes reagents required for PCR amplification/detection, as well as validated reaction controls.
The JAK2 V617F Mutation Analysis Kit features a streamlined workflow, incorporating first-strand cDNA synthesis and real-time PCR amplification in a single reaction well.
The kit does not include reagents or columns for DNA isolation.
