NTRK Fusion Gene Detection Kit

Cat Number: NTRK-RT48

The NTRK Fusion Gene Detection Kit detects oncogenic NTRK1, NTRK2, and NTRK3 gene fusions, which drive tumor growth in various solid cancers and are associated with response to Trk inhibitor therapies.

48 tests per kit
2-hour turnaround time
Works with FFPE and fresh frozen samples
Detects 65 variants in 8 reactions
For research use (RUO) in the U.S and European Union

Categories: Colorectal Cancer, Lung Cancer, Melanoma Tags: NTRK1, NTRK2, NTRK3

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Gene fusions involving NTRK1, NTRK2, and NTRK3 are critical oncogenic drivers in a wide range of solid tumors, making them essential biomarkers for cancer diagnosis, prognosis, and targeted therapy selection. While these fusions are rare in common cancers such as lung and colorectal cancer (0.1%–2%), they are highly prevalent in certain rare tumors, including secretory breast carcinoma, secretory salivary gland carcinoma, and infantile fibrosarcoma (>75%).

NTRK gene fusions result from chromosomal rearrangements, where the 3′ region of an NTRK gene fuses with the 5′ region of a fusion gene partner. The 5′ fusion partner’s promoter drives the expression of the fusion gene, leading to the production of an oncogenic fusion protein. The identification of NTRK gene fusions is crucial, as samples with these alterations have shown exceptional responses to Trk inhibitor therapies which block Trk signaling to suppress tumor growth.

The NTRK Fusion Gene Detection Kit is a real-time qPCR-based assay designed for the rapid and sensitive detection of 65 known NTRK fusion variants from FFPE RNA samples. The assay employs gene-specific primers and fluorescent hydrolysis probes, enabling the precise amplification of fusion transcripts.

Using FAM-labeled probes for fusion detection and a CFO560/VIC-labeled internal control (TUBA1A), the test ensures reliable and high-quality results even in challenging sample types.

The NTRK Fusion Gene Detection Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.

EntroGenʼs NTRK Fusion Gene Detection Kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. This test includes reagents required for PCR amplification/detection, as well as validated reaction controls.

This kit includes primer/probe mix for detection of all variants mentioned above, one-step enzyme mix for cDNA synthesis and PCR detection, as well as validated controls.

Columns and reagents for RNA isolation are not included.