ctDNA EGFR Mutation Detection Kit

Cat Number: ctEGFR-48

The ctDNA EGFR Mutation Detection Kit is a real-time PCR-based assay that detects somatic EGFR mutations in exons 18, 19, 20, and 21 from circulating tumor DNA, providing actionable insights for targeted therapy in lung cancer when tumor tissue is unavailable.

48 tests per kit
2-hour turnaround time
7 Reactions per sample
For research use (RUO) in the U.S
For in vitro diagnostic use (IVD) in the European Union

Category: Lung Cancer Tag: EGFR

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The epidermal growth factor receptor (EGFR) is a membrane protein that plays a central role in transmitting signals from extracellular ligands to intracellular pathways, such as the Ras-Raf-MAPK cascade, which drive cell proliferation and survival. Mutations in EGFR, particularly in exons 18, 19, 20, and 21, are frequently found in lung cancer and other solid tumors and have been shown to predict response to EGFR-targeted therapies.

The ctDNA EGFR Mutation Detection Kit is an ultra-sensitive, real-time PCR-based assay designed for the specific and sensitive detection of somatic EGFR mutations from circulating tumor DNA (ctDNA) isolated from plasma. Using mutant-specific primers and fluorescent hydrolysis probes, the assay preferentially amplifies mutant sequences even in samples with a high proportion of wild-type DNA.

This highly reliable and efficient assay provides valuable insights into tumor genetics.

A summary of mutations detected in each reaction is shown in the table below:

Reaction	Mutation
1	Exon 19 Deletions
	T790M
	L858R
2	C797S
3	L861Q
4	S768I
5	G719X
6	Ex20InsGGT/CAC
7	Ex20Ins9 (c.2300_2308dup)

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The ctDNA EGFR Mutation Detection Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.

EntroGenʼs ctDNA EGFR Mutation Detection Kit requires a real-time PCR instrument capable of detecting VIC, FAM, ROX, and CY5 fluorescent probes.

This test includes reagents required for PCR amplification/detection, as well as validated reaction controls.

Columns and reagents for cell-free DNA isolation are not included. Cell-free DNA can be extracted using EntroGen’s Cell-Free DNA Isolation Kit.

	Implementation of a Real-Time PCR based plasma ctDNA analysis for detection of progressive disease acquired by EGFR T790M mediated TKI resistance in NSCLC patients – Jensen, S.G, et.al.
	Is liquid biopsy a good molecular monitoring tool? Detection of L858R and T790M EGFR mutations in circulating tumor DNA derived from NSCLC patient treated with gefitinib (case study) – Katarzyna, E, Nalejska, E., Lewandowska, M., et. al.
	Assessment of EGFR gene mutations in cf-DNA in monitoring of response to EGFR TKIs in patients with lung adenocarcinoma – Nicoś, Marcin, Wojas-Krawczyk, Kamila, et. al.
	Detection of somatic mutations in ctDNA derived from adenocarcinoma patients – EGFR tyrosine kinase inhibitor monitoring preliminary study – Lewandowska, Marzena Anna, Nalejska, Ewelina, et. al.