EGFR Mutation Analysis Kit

Cat Number: EGFR-RT52

The EGFR Mutation Analysis Kit is a highly sensitive real-time PCR assay that detects key EGFR mutations in exons 18–21, enabling accurate and efficient mutation profiling to guide targeted cancer therapy decisions.

1% Limit of Detection (LOD)
52 tests per kit
2-hour turnaround time
Works with FFPE and fresh frozen samples
8 Reactions per sample
Complementary EPAS analysis software
For research use (RUO) in the U.S
For in vitro diagnostic use (IVD) in the European Union

Category: Lung Cancer Tag: EGFR

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The epidermal growth factor receptor (EGFR) gene encodes a receptor tyrosine kinase that plays a central role in transmitting signals from extracellular ligands to downstream pathways involved in cell proliferation and survival. Mutations in EGFR, particularly in exons 18, 19, 20, and 21, are frequently found in various solid tumors.

The EGFR Mutation Analysis Kit is a real-time PCR-based assay designed for the specific and sensitive detection of somatic EGFR mutations. Using mutant-specific primers and fluorescent hydrolysis probes, the assay preferentially amplifies mutant DNA even in samples with a high proportion of wild-type DNA.

This highly reliable and efficient assay provides valuable insights into tumor genetics.

EntroGen’s EGFR Mutation Analysis Kit detects the following EGFR mutations:

Exon 18	Exon 19	Exon 20	Exon 21
G719A – 2156G>C	2235-2249 del 15	T790M – 2369C>T	L858R – 2573T>G
G719S – 2155G>A	2235-2252>AAT del 18	S768I – 2303G>T	L861Q – 2582T>A
G719C – 2155G>T	2236-2253 del 18	2307-2308 ins GCCAGCGTG
G719D – 2156G>A	2237-2251 del 15	2319-2320 ins CAC
	2237-2254 del 18	2310-2311 ins GGT
	2237-2255>T del 19
	2236-2250 del 15
	2238-2255 del 18
	2238-2248>GC del 11
	2238-2252>GCA del 15
	2233-2247 del 15
	2234-2248 del 15
	2235-2246 del 12
	2235-2248>AATTC
	2235-2251>AATTC
	2235-2252>AAT
	2235-2255>AAT
	2236-2248>AGAC
	2236-2248>CAAC
	2236-2256 del 21
	2237-2252>T
	2239-2247 del 9
	2239-2256 del 18
	2239-2248>C del 10
	2239-2258>CA del 20
	2240-2251 del 12
	2240-2257 del 18
	2240-2254 del 15
	2239-2251>C del 13
	2237-2253>TC
	2237-2253>TTCCT
	2237-2253>TTGCT
	2237-2256>TC
	2237-2257>TCT
	2238-2252 del 15
	2239-2252>CA
	2239-2256>CAA
	2239-2262 del 24
	2248-2273>CC
	2252-2275 del 24
	2252-2276>A
	2252-2277>AT
	2253-2276 del 24
	2254-2277 del 24

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The EGFR Mutation Analysis Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.

EntroGenʼs EGFR Mutation Analysis Kit requires a real-time PCR instrument capable of detecting VIC and FAM fluorescent probes.

This test includes reagents required for PCR amplification/detection, as well as validated reaction controls.

Columns and reagents for DNA isolation are not included. Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA® Genomic DNA Isolation Kit.

We suggest using the Internal Quality Control Assay (cat. no. IQCA–RT50) to determine the optimal amount of input DNA prior to running this assay.

	Cytology smears as diagnostic material for EGFR gene testing in non-small cell lung cancer. Powrózek T, Krawczyk P, Pankowski J, Reszka K, Jakubiak M, Obrochta A, Wojas-Krawczyk K, Buczkowski J, Milanowski J. Tumori. 2015 Nov 14;101(6):e151-3. Pubmed ID 26108238
	The Use of a Two-Tiered Testing Strategy for the Simultaneous Detection of Small EGFR Mutations and EGFR Amplification in Lung Cancer. Lewandowska MA, Czubak K, Klonowska K, Jozwicki W, Kowalewski J, Kozlowski P. PLoS One. 2015 Feb 26;10(2):e0117983. Pubmed ID 25719557
	Borderline Brenner tumor of the ovary: a case report with immunohistochemical and molecular study. Rossella De Cecio, Monica Cantile, Francesca Collina, Laura Marra, Clemente Santonastaso, Cono Scaffa, Gerardo Botti, Nunzia Simona Losito. J Ovarian Res. 2014 Oct 29;7:101. Pubmed ID 25358264
	Discrepancies between ALK protein disruption and occurrence of ALK gene rearrangements in Polish NSCLC patients. Grenda A, Bożena J, Paweł K, et. al. J Thorac Dis. 2018 Aug; 10(8): 4994–5009. Pubmed ID 30233874
	EPAS (EntroGen Variant Analysis Software) Data Analysis