EGFRX Mutation Analysis Extension Kit

Cat Number: EGFRX-RT52

The EGFRX Mutation Analysis Extension Kit expands the mutation coverage of EntroGen’s EGFR-RT52 assay by enabling sensitive detection of rare EGFR mutations in codons L858, L861, and E709.

1% Limit of Detection (LOD)
52 tests per kit
2-hour turnaround time
Works with FFPE and fresh frozen samples
3 Reactions per sample
For research use (RUO) in the U.S
For in vitro diagnostic use (IVD) in the European Union

Category: Lung Cancer Tag: EGFR

Request More Information

The EGFRX Mutation Analysis Extension Kit is a real-time PCR-based add-on assay designed to expand the mutation detection capabilities of EntroGen’s EGFR-RT52 kit by targeting additional rare somatic mutations in EGFR, specifically affecting amino acids L858, L861, and E709.

This extension kit detects L858M and L861R with allele-specific precision and identifies E709 mutations (including E709K, E709V, and E709Q) through cross-reactivity at higher DNA inputs, all under the same conditions as the parent EGFR-RT52 assay.

Used in conjunction with the EGFR-RT52 kit, this add-on enhances the breadth of mutation coverage and supports more comprehensive EGFR profiling in solid tumor samples.

The EGFRX Mutation Analysis Extension Kit is a real-time PCR-based assay designed for the specific and sensitive detection of somatic EGFR mutations. Using mutant-specific primers and fluorescent hydrolysis probes, the assay preferentially amplifies mutant DNA even in samples with a high proportion of wild-type DNA.

EntroGen’s EGFRX Mutation Analysis Extension Kit detects the following EGFR mutations:

Amino Acid Affected	Amino Acid Change	Nucleotide Change	HGVS Nucleotide Change	HGVS Protein Change	Cosmic ID
L858	L858M	c.2572 C>A	LRG_304t1:c.2572C>A	LRG_304p1:p.(Leu858Met)	12366
L861	L861R	c.2582 T>G	LRG_304t1:c.2582T>G	LRG_304p1:p.(Leu861Arg)	12374
E709	E709A	c.2126 A>C	LRG_304t1:c.2126A>C	LRG_304p1:p.(Glu709Ala)	13427
	E709D	c.2127 A>C	LRG_304t1:c.2127A>C	LRG_304p1:p.(Glu709Asp)	N/A
	E709D	c.2127 A>T	LRG_304t1:c.2127A>T	LRG_304p1:p.(Glu709Asp)	N/A
	E709G	c.2126 A>G	LRG_304t1:c.2126A>G	LRG_304(p1):p.(Glu709Gly)	13009

For More Information

The EGFRX Mutation Analysis Extension Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a far less complicated process.

EntroGenʼs EGFRX Mutation Analysis Extension Kit requires a real-time PCR instrument capable of detecting VIC and FAM fluorescent probes.

This test includes reagents required for PCR amplification/detection, as well as validated reaction controls.

Columns and reagents for DNA isolation are not included. Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA® Genomic DNA Isolation Kit.

	Cytology smears as diagnostic material for EGFR gene testing in non-small cell lung cancer. Powrózek T, Krawczyk P, Pankowski J, Reszka K, Jakubiak M, Obrochta A, Wojas-Krawczyk K, Buczkowski J, Milanowski J. Tumori. 2015 Nov 14;101(6):e151-3. Pubmed ID 26108238
	The Use of a Two-Tiered Testing Strategy for the Simultaneous Detection of Small EGFR Mutations and EGFR Amplification in Lung Cancer. Lewandowska MA, Czubak K, Klonowska K, Jozwicki W, Kowalewski J, Kozlowski P. PLoS One. 2015 Feb 26;10(2):e0117983. Pubmed ID 25719557
	Borderline Brenner tumor of the ovary: a case report with immunohistochemical and molecular study. Rossella De Cecio, Monica Cantile, Francesca Collina, Laura Marra, Clemente Santonastaso, Cono Scaffa, Gerardo Botti, Nunzia Simona Losito. J Ovarian Res. 2014 Oct 29;7:101. Pubmed ID 25358264
	Discrepancies between ALK protein disruption and occurrence of ALK gene rearrangements in Polish NSCLC patients. Grenda A, Bożena J, Paweł K, et. al. J Thorac Dis. 2018 Aug; 10(8): 4994–5009. Pubmed ID 30233874
	EPAS (EntroGen Variant Analysis Software) Data Analysis