Library Quantification for Illumina
Cat Number: LIBQ-NGS
The Library Quantification for Illumina® Kit is intended for the assessment of library quality for downstream Illumina® sequencing.
- qPCR is the most accurate method for quantifying a sequencing library.
- Qualifies and quantifies library molecules that can be sequenced in downstream Illumina assays.
- Predicts library input for maximal and reliable data output.
- Simple set up and interpretation.
- Automated analysis available
- For research use (RUO) in the U.S
- For in vitro diagnostic use (IVD) in the European Union
The Library Quantification Kit for Illumina® is a qPCR-based assay designed to accurately quantify DNA libraries prepared for sequencing on Illumina platforms.
By specifically amplifying adapter-ligated DNA fragments, the assay provides precise and reproducible quantification to ensure optimal cluster generation and sequencing performance.
The kit includes pre-diluted DNA standards, primer mixes, and a 2X qPCR master mix with intercalating dye, allowing for streamlined setup and consistent results.
Compatible with most real-time PCR instruments, this kit enables reliable library normalization across runs, reducing variability and maximizing data quality in high-throughput NGS workflows.
The Library Quantification Kit for Illumina® features a simple and reliable workflow for accurate NGS library quantification. The assay uses qPCR to selectively amplify adapter-ligated fragments, ensuring that only sequence-ready libraries are measured.
This assay requires a real-time PCR instrument.
The kit includes:
- SYBR™ Green reaction mix.
- Primers that recognize p5 and p7 adaptor-sequences.
- 5 standards intended to generate a standard curve for determining library concentration.
This kit is for use with EntroGen’s NGS assays only. May be purchased separately. It is also supplied with the following kits:
The reagents in the kit are sufficient for 75 triplicate reactions including all standards, controls, and samples.
After preparing your libraries and diluting them to the recommended concentration, qPCR is performed using the provided 2X master mix, primer mix, and pre-diluted standards. The resulting Ct values are used to calculate the concentration of each library by comparison to the standard curve. The process enables accurate normalization of libraries for consistent cluster densities and optimal sequencing performance on Illumina® platforms.
Reagents for DNA library preparation are not included.
