DNA Fragmentation Quantification Assay
Cat Number: FQA-RT50
The DNA Fragmentation Quantification Assay is used to assess the quality and quantity of amplifiable DNA available for downstream applications like qPCR.
- Assesses sample quality by measuring fragmentation ratio.
- Quantifies amount of amplifiable DNA for downstream qPCR and NGS assays.
- Predicts optimal DNA input for maximal and reliable data output in qPCR and NGS.
- Simple set up and interpretation.
- Automated analysis available
- For research use (RUO) in the U.S
- For in vitro diagnostic use (IVD) in the European Union
The DNA Fragmentation Quantification Assay is a qPCR-based assay designed to evaluate the quality and amplifiability of DNA extracted from FFPE and fresh/frozen tissue samples. By amplifying three different fragment sizes (37 bp, 150 bp, and 300 bp) from a reference gene on chromosome 5, the assay provides a comprehensive assessment of DNA integrity.
Each sample is tested against a standard curve, yielding three key outputs:
- DNA concentration (ng/μl)
- Amplifiable copy numbers for each fragment size
- A calculated fragmentation ratio (F-ratio).
These values allow researchers to assess DNA suitability for downstream PCR-based applications and to normalize input amounts more accurately than traditional quantification methods.
The DNA Fragmentation Quantification Assay is a simple and efficient qPCR-based assay used to assess the integrity and amplifiability of FFPE-derived DNA. It evaluates fragmentation by amplifying three target sizes—37 bp, 150 bp, and 300 bp—from a reference gene using SYBR® Green detection chemistry. Results are available in under 2 hours.
EntroGen’s DNA Fragmentation Quantification Assay includes primer mixes, standards, and reagents needed for PCR amplification, along with analysis templates for automated interpretation.
Reagents and columns for DNA isolation are not included.
