Thyroid Cancer Mutation Detection Kit
Cat Number: THDNA-RT64
The Thyroid Cancer Mutation Detection Kit is a PCR-based assay that detects somatic mutations in the BRAF, KRAS, NRAS, and HRAS genes, which are associated with thyroid cancer development, prognosis, and treatment selection.
- 64 tests per kit
- 2-hour turnaround time
- Works with FFPE, fresh frozen and FNA samples
- 5 Reactions per sample
- For research use (RUO) in the U.S
- For in vitro diagnostic use (IVD) in the European Union
Mutations in the BRAF, KRAS, NRAS, and HRAS genes are frequently found in thyroid cancer, the most common endocrine malignancy in the United States. These genetic alterations play a key role in tumor initiation and progression, helping to distinguish malignant thyroid tumors from benign nodules.
The Thyroid Cancer Mutation Detection Kit uses real-time PCR to accurately detect somatic mutations in the BRAF, KRAS, NRAS, and HRAS genes, even in samples containing a mixture of mutant and wild-type DNA. The Thyroid Cancer Mutation Detection Kit is a qPCR assay designed to detect key thyroid cancer mutations with high sensitivity.
The kit identifies the presence of the mutations in a total of 5 reactions per sample.
| Gene | Amino Acid Change | Nucleotide Change |
HGVS AA Change | HGVS nt Change | Cosmic ID | Detected by Primer No. |
| BRAF | V600E | c.1799T>A | LRG_299p1:p.(Val600Glu) | LRG_299t1:c.1799T>A | 476 | 1 |
| KRAS | G12A | c.35G>C | LRG_344p1:p.(Gly12Ala) | LRG_344t1:c.35G>C | 522 | 2 |
| G12D | c.35G>A | LRG_344p1:p.(Gly12Asp) | LRG_344t1:c.35G>A | 521 | ||
| G12R | c.34G>C | LRG_344p1:p.(Gly12Arg) | LRG_344t1:c.34G>C | 518 | ||
| G12V | c.35G>T | LRG_344p1:p.(Gly12Val) | LRG_344t1:c.35G>T | 520 | ||
| G13D | c.38G>A | LRG_344p1:p.(Gly13Asp) | LRG_344t1:c.38G>A | 532 | ||
| G12C | c.34G>T | LRG_344p1:p.(Gly12Cys) | LRG_344t1:c.34G>T | 516 | 3 | |
| G12S | c.34G>A | LRG_344p1:p.(Gly12Ser) | LRG_344t1:c.34G>A | 517 | ||
| NRAS | Q61H (CAC) | c.183A>C | LRG_92p1:p.(Gln61His) | LRG_92t1:c.183A>C | 586 | 4 |
| Q61H (CAT) | c.183A>T | LRG_92p1:p.(Gln61His) | LRG_92t1:c.183A>T | 585 | ||
| Q61L | c.182A>T | LRG_92p1:p.(Gln61Leu) | LRG_92t1:c.182A>T | 583 | ||
| Q61K | c.181C>A | LRG_92p1:p.(Gln61Lys) | LRG_92t1:c.181C>A | 580 | ||
| Q61R | c.182A>G | LRG_92p1:p.(Gln61Arg) | LRG_92t1:c.182A>G | 584 | ||
| HRAS | G12V | c.35G>T | LRG_506p1:p.(Gly12Val) | LRG_506t1:c.35G>T | 483 | 5 |
| 5G13R | c.37G>C | LRG_506p1:p.(Gly13Arg) | LRG_506t1:c.37G>C | 486 | ||
| Q61R | c.182A>G | LRG_506p1:p.(Gln61Arg) | LRG_506t1:c.182A>G | 499 |
For More Information
The Thyroid Cancer Mutation Detection Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.
EntroGenʼs Thyroid Cancer Mutation Detection Kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. This test includes reagents required for PCR amplification/detection, as well as validated reaction controls.
Columns and reagents for DNA isolation are not included. Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA® Genomic DNA Isolation Kit.
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