{"id":15,"date":"2015-01-06T19:48:44","date_gmt":"2015-01-06T19:48:44","guid":{"rendered":"http:\/\/entrogen.com\/web4\/?page_id=15"},"modified":"2022-10-14T21:00:27","modified_gmt":"2022-10-14T21:00:27","slug":"b-raf-codon-600-mutation-analysis-kit","status":"publish","type":"page","link":"https:\/\/entrogen.com\/web4\/b-raf-codon-600-mutation-analysis-kit\/","title":{"rendered":"BRAF Codon 600 Mutation Analysis Kit II"},"content":{"rendered":"[et_pb_section fullwidth=”on” specialty=”off”][et_pb_fullwidth_header admin_label=”Fullwidth Header” title=”BRAF Codon 600 Mutation Analysis Kit II” background_layout=”dark” text_orientation=”left” module_class=”product-page-header” \/][\/et_pb_section][et_pb_section][et_pb_row][et_pb_column type=”2_3″][et_pb_text admin_label=”Text” background_layout=”light” text_orientation=”left”]\n
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Available Products<\/h4>\n\n\n\n\n
Product Name\/Description <\/strong><\/th>\n\n
No. of Reactions* <\/strong><\/div>\n<\/th>\n
Product Code <\/strong><\/th>\n<\/tr>\n
BRAF Codon 600 Mutation Analysis Kit II for Real-Time PCR (V600E\/K\/D\/R\/M\/G)<\/td>\n\n
64<\/div>\n<\/td>\n
BRAFX-RT64<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

*Includes all controls.<\/p>\n

<\/h4>\n

BRAF<\/em> Mutations and Cancer<\/h4>\n

The BRAF<\/em> gene encodes a serine\/threonine protein kinase, which plays a role in regulating the MAP Kinase\/ERK signaling pathways, affecting cell growth and proliferation. Somatic mutations in this gene have been found in cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, papillary thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung.The most common mutations in BRAF<\/em> occur in codon 600, where an amino acid substitution in the activation segment of the kinase domain creates a constitutively active form of the protein. BRAF<\/em> mutations are generally found in tumors that are wild-type for KRAS, NRAS, and EGFR.<\/p>\n

<\/h4>\n

Testing Procedure and Analysis<\/h4>\n

The table below lists the mutations detected by this kit and the approximate frequencies found in various cancers.<\/p>\n\n\n\n\n\n\n\n\n\n
Mutation Name<\/th>\nNucleotide Change<\/th>\nAmino Acid Change<\/th>\nFrequency<\/th>\n<\/tr>\n
V600E<\/td>\nc.1799T>A<\/td>\nValine (V) to glutamic acid (E)<\/td>\n70-90%<\/td>\n<\/tr>\n
V600K<\/td>\nc.1798_1799GT>AA<\/td>\nValine (V) to lysine acid (K)<\/td>\n10-15%<\/td>\n<\/tr>\n
V600D<\/td>\nc.1799_1800TG>AT<\/td>\nValine (V) to aspartic acid (D)<\/td>\n<5%<\/td>\n<\/tr>\n
V600R<\/td>\nc.1798_1799GT>AG<\/td>\nValine (V) to arginine (R)<\/td>\n<5%<\/td>\n<\/tr>\n
V600M<\/td>\nc.1798G>A<\/td>\nValine (V) to methionine (M)<\/td>\n<1%<\/td>\n<\/tr>\n
V600G<\/td>\nc.1799T>G<\/td>\nValine (V) to Glycine(G)<\/td>\n<1%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

EntroGen\u2019s BRAF<\/em> codon 600 mutation analysis kit is a polymerase chain reaction (PCR)-based assay that uses allele-specific probes to identify the presence of the BRAF<\/em>\u00a0 V600E, V600K, V600D, V600R, V600M and V600G mutations. The testing procedure involves three (3) simple steps:<\/p>\n