{"id":4228,"date":"2025-03-17T16:32:53","date_gmt":"2025-03-17T16:32:53","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4228"},"modified":"2025-06-23T18:12:01","modified_gmt":"2025-06-23T18:12:01","slug":"braf-codon-600-mutation-analysis-kit-ii","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/braf-codon-600-mutation-analysis-kit-ii\/","title":{"rendered":"BRAF Codon 600 Mutation Analysis Kit II"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images admin_label=”Woo Product Images” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” global_colors_info=”{}”][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

Mutations in the BRAF gene, particularly in codon 600 of exon 15, are commonly found in cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, papillary thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. Studies show that samples with these mutations may benefit from targeted kinase inhibitor therapies, highlighting the importance of comprehensive mutation testing for informing therapeutic strategies.<\/p>\n

The BRAF Codon 600 Mutation analysis real-time assay uses real-time PCR to accurately detect somatic mutations in exon 15 of the BRAF gene, even in samples with mostly normal DNA.\u00a0<\/p>\n

The kit identifies the presence of the mutations in a total of 5 reactions per sample.<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n
Mutation Name<\/strong><\/td>\nNucleotide Change<\/strong><\/td>\nAmino Acid Change<\/strong><\/td>\nFrequency<\/strong><\/td>\n<\/tr>\n
V600E<\/td>\nc.1799T>A<\/td>\nValine (V) to glutamic acid (E)<\/td>\n70-90%<\/td>\n<\/tr>\n
V600K<\/td>\nc.1798_1799GT>AA<\/td>\nValine (V) to lysine acid (K)<\/td>\n10-15%<\/td>\n<\/tr>\n
V600D<\/td>\nc.1799_1800TG>AT<\/td>\nValine (V) to aspartic acid (D)<\/td>\n<5%<\/td>\n<\/tr>\n
V600R<\/td>\nc.1798_1799GT>AG<\/td>\nValine (V) to arginine (R)<\/td>\n<5%<\/td>\n<\/tr>\n
V600M<\/td>\nc.1798G>A<\/td>\nValine (V) to methionine (M)<\/td>\n<1%<\/td>\n<\/tr>\n
V600G<\/td>\nc.1799T>G<\/td>\nValine (V) to Glycine(G)<\/td>\n<1%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The BRAF Mutation Analysis Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.<\/p>\n

EntroGen\u02bcs BRAF mutation screening panel requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. This test includes reagents required for PCR amplification\/detection, as well as validated reaction controls.<\/p>\n

Columns and reagents for DNA isolation are not included. Genomic DNA from frozen or formalin-fixed paraffin-embedded (FFPE) tissues can be extracted using commercial DNA extraction kit, such as the EntroGen <\/a>pureNA\u00ae Genomic DNA Isolation Kit<\/a>. Cell-free DNA from plasma can be extracted using commercial DNA extraction kit, such as the EntroGen pureNA\u00ae Cell-Free DNA Isolation Kit<\/a>.<\/p>\n

\"\"\"\" \"\"\"\" \"\"<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

<\/a><\/p>\n\n\n\n
\"\"<\/a><\/strong><\/td>\nBRAF Testing in Multifocal Papillary Thyroid Carcinoma<\/strong>
Kimbrell HZ, Sholl AB, Ratnayaka S, Japa S, Lacey M, Carpio G, Bhatia P, Kandil E. Biomed Res Int.\u00a0 2015;2015:486391.Pubmed ID 26448939<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n

 <\/p>\n

 <\/p>\n[\/et_pb_tab][\/et_pb_tabs][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_related_products include_categories=”current” show_price=”off” show_rating=”off” show_sale_badge=”off” _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_wc_related_products][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_section]\n","protected":false},"excerpt":{"rendered":"

Cat Number: BRAFX-RT64<\/strong><\/p>\n

The BRAF Codon 600 Mutation Analysis Kit is a PCR-based assay that detects somatic mutations in exon 15 of the BRAF gene, which are associated with cancer growth and progression, particularly in\u00a0 advanced melanoma.<\/p>\n

 <\/p>\n