{"id":4277,"date":"2025-03-26T20:08:35","date_gmt":"2025-03-26T20:08:35","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4277"},"modified":"2025-06-23T17:19:50","modified_gmt":"2025-06-23T17:19:50","slug":"colorectal-cancer-mutation-detection-panel-for-real-time-pcr","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/colorectal-cancer-mutation-detection-panel-for-real-time-pcr\/","title":{"rendered":"Colorectal Cancer Mutation Detection Panel for Real-Time PCR"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” global_colors_info=”{}”][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

Mutations in the KRAS, NRAS, BRAF, PIK3CA, and AKT1 genes are frequently found in colorectal cancer and play a significant role in tumor progression and resistance to anti-EGFR therapies such as cetuximab and panitumumab. Identifying these mutations is essential for understanding tumor biology and guiding research on targeted treatment strategies.<\/p>\n

The Colorectal Cancer Mutation Detection Panel uses real-time PCR to accurately detect somatic mutations in KRAS, NRAS, BRAF, PIK3CA, and AKT1, even in samples containing a mixture of mutant and wild-type DNA. The assay utilizes allele-specific primers and fluorescent probes for reliable mutation identification and includes an internal control to ensure the integrity of the DNA and reagents.<\/p>\n

The Colorectal Cancer Mutation Detection Panel is a qPCR assay designed to detect multiple mutations in a total of 6 reactions per sample, providing a comprehensive genetic profile to support colorectal cancer research.<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene<\/strong><\/td>\nExon<\/strong><\/td>\nAmino Acid Change<\/strong><\/td>\nNucleotide Change<\/strong><\/td>\nCosmic ID<\/strong><\/td>\nReaction No.<\/strong><\/td>\n<\/tr>\n
KRAS<\/strong><\/td>\n2<\/td>\nG12A<\/td>\nc.35G>C<\/td>\n522<\/td>\n1<\/td>\n<\/tr>\n
G12D<\/td>\nc.35G>A<\/td>\n521<\/td>\n1<\/td>\n<\/tr>\n
G12R<\/td>\nc.34G>C<\/td>\n518<\/td>\n1<\/td>\n<\/tr>\n
G12C<\/td>\nc.34G>T<\/td>\n516<\/td>\n1<\/td>\n<\/tr>\n
G12S<\/td>\nc.34G>A<\/td>\n517<\/td>\n1<\/td>\n<\/tr>\n
G12V<\/td>\nc.35G>T<\/td>\n520<\/td>\n1<\/td>\n<\/tr>\n
G13D<\/td>\nc.38G>A<\/td>\n532<\/td>\n1<\/td>\n<\/tr>\n
3<\/td>\nA59T<\/td>\nc.175G>A<\/td>\n546<\/td>\n6<\/td>\n<\/tr>\n
A59E<\/td>\nc.176C>A<\/td>\n547<\/td>\n6<\/td>\n<\/tr>\n
A59G<\/td>\nc.176C>G<\/td>\n28518<\/td>\n6<\/td>\n<\/tr>\n
Q61H<\/td>\nc.183A>C<\/td>\n554<\/td>\n4<\/td>\n<\/tr>\n
Q61H<\/td>\nc.183A>T<\/td>\n555<\/td>\n4<\/td>\n<\/tr>\n
Q61L<\/td>\nc.182A>T<\/td>\n553<\/td>\n4<\/td>\n<\/tr>\n
Q61R<\/td>\nc.182A>G<\/td>\n552<\/td>\n4<\/td>\n<\/tr>\n
4<\/td>\nK117N<\/td>\nc.351A>C<\/td>\n19940<\/td>\n1<\/td>\n<\/tr>\n
K117N<\/td>\nc.351A>T<\/td>\n28519<\/td>\n1<\/td>\n<\/tr>\n
K117R<\/td>\nc.350A>G<\/td>\n4696722<\/td>\n1<\/td>\n<\/tr>\n
K117E<\/td>\nc.349A>G<\/td>\n\u2014<\/td>\n1<\/td>\n<\/tr>\n
A146T<\/td>\nc.436G>A<\/td>\n19404<\/td>\n5<\/td>\n<\/tr>\n
A146P<\/td>\nc.436G>C<\/td>\n19905<\/td>\n5<\/td>\n<\/tr>\n
A146V<\/td>\nc.437C>T<\/td>\n19900<\/td>\n5<\/td>\n<\/tr>\n
NRAS<\/strong><\/td>\n2<\/td>\nG12D<\/td>\nc.35G>A<\/td>\n564<\/td>\n2<\/td>\n<\/tr>\n
G12S<\/td>\nc.34G>A<\/td>\n563<\/td>\n2<\/td>\n<\/tr>\n
G12C<\/td>\nc.34G>T<\/td>\n562<\/td>\n2<\/td>\n<\/tr>\n
G13R<\/td>\nc.37G>C<\/td>\n569<\/td>\n2<\/td>\n<\/tr>\n
G13V<\/td>\nc.38G>T<\/td>\n574<\/td>\n2<\/td>\n<\/tr>\n
3<\/td>\nA59T<\/td>\nc.175G>A<\/td>\n578<\/td>\n6<\/td>\n<\/tr>\n
A59D<\/td>\nc.176C>A<\/td>\n253327<\/td>\n6<\/td>\n<\/tr>\n
Q61K<\/td>\nc.181C>A<\/td>\n580<\/td>\n3<\/td>\n<\/tr>\n
Q61L<\/td>\nc.182A>T<\/td>\n583<\/td>\n3<\/td>\n<\/tr>\n
Q61R<\/td>\nc.182A>G<\/td>\n584<\/td>\n3<\/td>\n<\/tr>\n
Q61H<\/td>\nc.183A>C<\/td>\n586<\/td>\n3<\/td>\n<\/tr>\n
Q61H<\/td>\nc.183A>T<\/td>\n585<\/td>\n3<\/td>\n<\/tr>\n
4<\/td>\nK117R<\/td>\nc.350A>G<\/td>\n\u2014<\/td>\n2<\/td>\n<\/tr>\n
A146T<\/td>\nc.436G>A<\/td>\n27174<\/td>\n5<\/td>\n<\/tr>\n
BRAF<\/strong><\/td>\n15<\/td>\nV600E<\/td>\nc.1799T>A<\/td>\n476<\/td>\n3<\/td>\n<\/tr>\n
V600E2<\/td>\nc.1799_1800TG>AA<\/td>\n\u2014<\/td>\n3<\/td>\n<\/tr>\n
V600D<\/td>\nc.1799_1800TG>AT<\/td>\n477<\/td>\n3<\/td>\n<\/tr>\n
V600K<\/td>\nc.1798_1799GT>AA<\/td>\n473<\/td>\n3<\/td>\n<\/tr>\n
PIK3CA<\/strong><\/td>\n9<\/td>\nE542K<\/td>\nc.1624G>A<\/td>\n760<\/td>\n4<\/td>\n<\/tr>\n
E545K<\/td>\nc.1633G>A<\/td>\n763<\/td>\n4<\/td>\n<\/tr>\n
E545Q<\/td>\nc.1633G>C<\/td>\n27133<\/td>\n4<\/td>\n<\/tr>\n
20<\/td>\nH1047R<\/td>\nc.3140A>G<\/td>\n775<\/td>\n4<\/td>\n<\/tr>\n
H1047L<\/td>\nc.3140A>T<\/td>\n776<\/td>\n4<\/td>\n<\/tr>\n
AKT1<\/strong><\/td>\n4<\/td>\nE17K<\/td>\nc.49G>A<\/td>\n33765<\/td>\n6<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

Colorectal Cancer Mutation Detection Panel follows a simple and easy to use process. It can be completed in 2 hours and follows a far less complicated process.<\/p>\n

EntroGen\u02bcs Colorectal cancer mutation screening panel requires a real-time PCR instrument capable of detecting FAM, ROX, CY5 and VIC fluorescent probes. This test includes reagents required for PCR amplification\/detection, as well as validated reaction controls.<\/p>\n

Columns and reagents for DNA isolation are not included.\u00a0Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA\u00ae Genomic DNA Isolation Kit<\/a>.<\/p>\n

We suggest using the <\/span>Internal Quality Control Assay (cat. no. IQCA<\/span><\/span>–<\/span><\/a>RT50)<\/a> to determine the optimal amount of input DNA prior to runnin<\/span>g this<\/span> assay.<\/span><\/span><\/p>\n

\"\"\u00a0\"\" \"\"\u00a0 \u00a0\u00a0\"\" \u00a0\u00a0\"\"<\/p>\n

<\/h5>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\"\"<\/a><\/td>\nFrequency and type of KRAS mutations in routine diagnostic analysis of metastatic colorectal cancer
<\/strong>Jens Neumann, Evelyn Zeindl-Eberhart, Thomas Kirchner, Andreas Jung. Pubmed ID 19679400<\/span><\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nHigh frequency of mutations of the PIK3CA gene in human cancers
<\/strong>Yardena Samuels et al. Pubmed ID 15016963<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nBRAF mutation as a biomarker in colorectal cancer
<\/strong>Varghese AM, Saltz L<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nPrognostic and Predictive Biomarkers in Resected Colon Cancer: Current Status and Future Perspectives for Integrating Genomics into Biomarker Discovery
<\/strong>Sabine Tejpar et al. Pubmed ID 20350999<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nEffects of KRAS, BRAF, NRAS, and PIK3CA mutations on the efficacy of cetuximab plus chemotherapy in chemotherapy-refractory metastatic colorectal cancer: a retrospective consortium analysis
<\/strong>Wendy De Roock et a. Pubmed ID 20619739<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nNRAS mutations are rare in colorectal cancer
<\/strong>Natsumi Irahara et al.\u00a0 Pubmed ID: 20736745<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nFrequency of KRAS, BRAF, and NRAS mutations in colorectal cancer
<\/strong>Cecily P Vaughn , Scott D Zobell, Larissa V Furtado, Christine L Baker, Wade S Samowitz. Pubmed ID\u00a021305640<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nA rapid, sensitive, reproducible and cost-effective method for mutation profiling of colon cancer and metastatic lymph nodes
<\/strong>Debora Fumagalli 1 , Patrick G Gavin, Yusuke Taniyama, Seung-Il Kim, Hyun-Joo Choi, Soonmyung Paik, Katherine L Pogue-Geile. Pubmed ID 20233444<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nMutational analysis of oncogenic AKT E17K mutation in common solid cancers and acute leukaemias<\/strong> <\/a>
M S Kim 1 , E G Jeong, N J Yoo, S H Lee. Pubmed ID 18392055<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n

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Cat Number: CRC-RT48<\/strong><\/p>\n

The Colorectal Cancer Mutation Detection Panel is a PCR-based assay that detects somatic mutations in the KRAS, NRAS, BRAF, PIK3CA, and AKT1 genes, which are associated with tumor progression and resistance to anti-EGFR therapies in colorectal cancer.<\/p>\n

 <\/p>\n