{"id":4279,"date":"2025-03-26T21:01:27","date_gmt":"2025-03-26T21:01:27","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4279"},"modified":"2025-11-19T00:14:39","modified_gmt":"2025-11-19T00:14:39","slug":"idh1-2-mutation-detection-kit","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/idh1-2-mutation-detection-kit\/","title":{"rendered":"IDH1\/2 Mutation Detection Kit"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

Mutations in the IDH1 and IDH2 genes are among the most significant genetic alterations in diffuse gliomas and serve as key biomarkers for tumor classification, prognosis, and treatment planning. Studies have shown that IDH1\/2 mutations are highly prevalent in lower-grade gliomas (WHO grades II and III) and secondary glioblastomas, where they are associated with better patient survival outcomes. Additionally, these mutations are known to influence tumor metabolism, making them potential targets for emerging therapeutic strategies.<\/p>\n

In addition to their role in gliomas, IDH1\/2 mutations are found in approximately 20% of acute myeloid leukemia (AML) patients. These mutations disrupt key cellular processes, preventing bone marrow cells from maturing properly and contributing to leukemia development. Targeting these mutations, IDH inhibitors have been developed to help leukemia cells differentiate into normal blood cells. Several of these targeted therapies have been approved for treating AML patients with specific IDH1\/2 mutations, highlighting the importance of comprehensive mutation testing for informing therapeutic strategies.<\/p>\n

The IDH1\/2 Mutation Detection Kit is a real-time PCR-based assay for accurate and sensitive mutation detection. This assay is designed to preferentially amplify mutant DNA, ensuring reliable results even in samples with a high proportion of wild-type DNA.<\/p>\n

The test provides a rapid and robust solution for identifying IDH1 and IDH2 mutations in FFPE and fresh\/frozen tissue samples.<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene<\/strong><\/td>\nExon<\/strong><\/td>\naa change<\/strong><\/td>\nnt change<\/strong><\/td>\nCosmic ID<\/strong><\/td>\nDetected by Primer No.<\/strong><\/td>\n<\/tr>\n
IDH1<\/td>\n4<\/td>\nR132H<\/td>\nc.395G>A<\/td>\nCOSM28746<\/td>\n1<\/td>\n<\/tr>\n
R132C<\/td>\nc.394C>T<\/td>\nCOSM28747<\/td>\n<\/tr>\n
R132S<\/td>\nc.394C>A<\/td>\nCOSM28748<\/td>\n<\/tr>\n
R132G<\/td>\nc.394C>G<\/td>\nCOSM28749<\/td>\n<\/tr>\n
R132L<\/td>\nc.395G>T<\/td>\nCOSM28750<\/td>\n<\/tr>\n
R132P<\/td>\nc.395G>C<\/td>\nCOSM221574<\/td>\n<\/tr>\n
R132V<\/td>\nc.394_395CG>GT<\/td>\nCOSM28751<\/td>\n<\/tr>\n
R100Q<\/td>\nc.299G>A<\/td>\nCOSM88208<\/td>\n2<\/td>\n<\/tr>\n
IDH2<\/td>\n4<\/td>\nR172K<\/td>\nc.515G>A<\/td>\nCOSM33733<\/td>\n3<\/td>\n<\/tr>\n
R172M<\/td>\nc.515G>T<\/td>\nCOSM33732<\/td>\n<\/tr>\n
R172W<\/td>\nc.514A>T<\/td>\nCOSM34039<\/td>\n<\/tr>\n
R172G<\/td>\nc.514A>G<\/td>\nCOSM33731<\/td>\n<\/tr>\n
R172S<\/td>\nc.516G>T<\/td>\nCOSM34090<\/td>\n<\/tr>\n
R140Q<\/td>\nc.419G>A<\/td>\nCOSM41590<\/td>\n4<\/td>\n<\/tr>\n
R140W<\/td>\nc.418C>T<\/td>\nCOSM41877<\/td>\n<\/tr>\n
R140L<\/td>\nc.419G>T<\/td>\nCOSM41875<\/td>\n5<\/td>\n<\/tr>\n
R140G<\/td>\nc.418C>G<\/td>\nCOSM1737874<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n For More Information<\/span><\/a>\n

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The IDH1\/2 Mutation Detection Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.<\/p>\n

EntroGen\u02bcs IDH1\/2 mutation detection kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. This test includes reagents required for PCR amplification\/detection, as well as validated reaction controls.<\/p>\n

Columns and reagents for DNA isolation are not included. Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA\u00ae Genomic DNA Isolation Kit<\/a>.<\/p>\n

\"\"\"\" \"\"\u00a0\"\" \"\"<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]\n

 <\/p>\n\n\n\n\n
\"\"<\/a><\/td>\nPrognostic and Predictive Biomarkers in Gliomas<\/strong>. \u015aledzi\u0144ska P, Bebyn MG, Furtak J, Kowalewski J, Lewandowska MA. Special Issue: Biomolecular Characterization of Brain Gliomas and Molecular Alterations Caused by Therapy. International Journal of Molecular Sciences. 2021, 22(19), 10373; DOI.org ID.<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\n\n

Glioma 2021 WHO Classification: The Superiority of NGS Over IHC in Routine Diagnostics.<\/strong> \u015aledzi\u0144ska, P., Bebyn, M., Szczerba, E. et al.<\/i> Mol Diagn Ther<\/i> 26<\/b>, 699\u2013713 (2022). https:\/\/doi.org\/10.1007\/s40291-022-00612-3<\/a><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Click to download lot-specific quality control data for your product:<\/p>\n

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Cat Number: IDH-RT38<\/strong><\/p>\n

The IDH1\/2 Mutation Detection Kit is a PCR-based test that identifies mutations in the IDH1 and IDH2 genes, key biomarkers in diffuse gliomas and acute myeloid leukemia (AML).<\/p>\n

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