{"id":4321,"date":"2025-03-28T22:22:22","date_gmt":"2025-03-28T22:22:22","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4321"},"modified":"2026-06-22T17:15:24","modified_gmt":"2026-06-22T17:15:24","slug":"jak2-v617f-mutation-analysis-kit","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/jak2-v617f-mutation-analysis-kit\/","title":{"rendered":"JAK2 V617F Mutation Analysis Kit"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” global_colors_info=”{}”][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The JAK2 V617F mutation results in a constitutively active JAK2 kinase, driving uncontrolled cellular proliferation and survival. This mutation arises from a valine (V) to phenylalanine (F) substitution at codon 617, causing JAK2 activation independent of upstream signals. The JAK2 V617F mutation has been identified in various malignancies and is associated with reduced sensitivity to JAK2 inhibitors, distinguishing it from other oncogenic driver mutations such as EGFR, KRAS, and ALK mutations.<\/p>\n

The MUT-RT50 JAK2 V617F Mutation Analysis Kit is a highly sensitive and specific real-time PCR-based assay designed to detect the JAK2 V617F mutation in DNA extracted from blood or bone marrow samples. The assay employs mutation-specific primers that preferentially amplify mutant DNA, ensuring highly specific detection even in a background of wild-type DNA.<\/p>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The JAK2 V617F Mutation Analysis Kit follows a simple and easy to use process. It can be completed in 2 hours.<\/p>\n

EntroGen\u02bcs JAK2 V617F Mutation Analysis Kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. This test includes reagents required for PCR amplification\/detection, as well as validated reaction controls.<\/p>\n

The JAK2 V617F Mutation Analysis Kit features a streamlined workflow, incorporating first-strand cDNA synthesis and real-time PCR amplification in a single reaction well.<\/p>\n

The kit does not include reagents or columns for DNA isolation.<\/p>\n

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Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n

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Cat Number: MUT-RT50<\/strong><\/p>\n

The MUT-RT50 JAK2 V617F Mutation Analysis Kit is a highly sensitive and specific molecular diagnostic tool designed for the detection of the JAK2 V617F mutation, optimized for assessing and guiding therapeutic decisions in various malignancies.<\/p>\n

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