{"id":4350,"date":"2025-04-01T22:29:32","date_gmt":"2025-04-01T22:29:32","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4350"},"modified":"2026-02-05T20:47:00","modified_gmt":"2026-02-05T20:47:00","slug":"c-kit-mutation-detection-kit","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/c-kit-mutation-detection-kit\/","title":{"rendered":"c-Kit Mutation Detection Kit"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.5″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

Mutations in the c-KIT gene are key drivers of tumorigenesis in various cancers, including gastrointestinal stromal tumors (GISTs), melanoma, and mastocytosis. These mutations lead to constitutive activation of the KIT receptor tyrosine kinase, promoting uncontrolled cell growth and survival.\u00a0<\/p>\n

The c-Kit Mutation Detection Kit is a real-time PCR-based assay designed for the rapid and sensitive detection of somatic mutations in the c-KIT gene. Using fluorescent hydrolysis probes, the assay accurately identifies mutant sequences even in samples with a high proportion of wild-type DNA.<\/p>\n

It includes an internal control gene to ensure assay reliability and is optimized for use with FFPE and fresh\/frozen tissue samples.<\/p>\n

The c-Kit Mutation Detection Kit detects the following mutations:<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Mutation<\/strong><\/td>\nCOSMIC ID<\/strong><\/td>\nPrimer Mix Tube<\/strong><\/td>\n<\/tr>\n
EX11_p.V559D<\/td>\nCOSM1252<\/td>\n1<\/td>\n<\/tr>\n
EX11_p.V559G<\/td>\nCOSM1253<\/td>\n<\/tr>\n
EX11_p.V560D<\/td>\nCOSM1257<\/td>\n<\/tr>\n
EX11_W557R<\/td>\nCOSM1216<\/td>\n<\/tr>\n
EX17_p.D816H<\/td>\nCOSM1311<\/td>\n<\/tr>\n
EX17_p.D816V<\/td>\nCOSM1314<\/td>\n<\/tr>\n
EX11_p.L576P<\/td>\nCOSM1290<\/td>\n2<\/td>\n<\/tr>\n
2EX13_p.K642E<\/td>\nCOSM1304<\/td>\n<\/tr>\n
EX9_p.Y503_F504insAY<\/td>\nCOSM12444<\/td>\n<\/tr>\n
EX11_p.W557_E561del<\/td>\nCOSM18890<\/td>\n3<\/td>\n<\/tr>\n
EX11_p.W557_K558del<\/td>\nCOSM12449<\/td>\n<\/tr>\n
EX11_p.W557_V559>C<\/td>\nCOSM1233<\/td>\n<\/tr>\n
EX11_p.W557_V559>F<\/td>\nCOSM1226<\/td>\n<\/tr>\n
EX17_p.N822Y<\/td>\nCOSM19109<\/td>\n<\/tr>\n
EX17_p.K818R<\/td>\nCOSM1315<\/td>\n<\/tr>\n
EX17_p.N822H<\/td>\nCOSM1318<\/td>\n<\/tr>\n
EX11_p.V559A<\/td>\nCOSM1255<\/td>\n4<\/td>\n<\/tr>\n
EX11_p.W557G<\/td>\nCOSM1221<\/td>\n<\/tr>\n
EX17_p.D820G<\/td>\nCOSM1316<\/td>\n<\/tr>\n
EX17_p.D820Y<\/td>\nCOSM12710<\/td>\n<\/tr>\n
EX17_p.N822K<\/td>\nCOSM1322<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n For More Information<\/span><\/a>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The c-KIT Mutation Detection Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.<\/p>\n

EntroGen\u02bcs c-KIT Mutation Gene Detection Kit requires a real-time PCR instrument capable of detecting FAM, ROX and CY5 fluorescent probes. This test includes reagents required for PCR amplification\/detection, as well as validated reaction controls.<\/p>\n

Columns and reagents for DNA isolation are not included.\u00a0Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA\u00ae Genomic DNA Isolation Kit<\/a>.<\/p>\n

\"\"\"\" \u00a0\"\"\"\" \"\"<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.5″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]\n\n\n\n\n\n\n
\"\"<\/a><\/td>\nImatinib for melanomas harboring mutationally activated or amplified KIT arising on mucosal, acral, and chronically sun-damaged skin
<\/strong>Hodi FS, Corless CL, Giobbie-Hurder A, et al. PMID 23775962<\/span><\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\n<\/a>KIT as a therapeutic target in metastatic melanoma<\/strong>. <\/a>Carvajal RD, Antonescu CR, Wolchok JD, et al.\u00a0 JAMA<\/em>. 2011;305(22):2327-2334<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nPhase II, open-label, single-arm trial of imatinib mesylate in patients with metastatic melanoma harboring c-Kit mutation or amplification<\/strong><\/a>
Guo J, Si L, Kong Y, et al. PMID 21690468<\/span><\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nPre-and Postoperative Circulating Tumour DNA in Patients With Gastrointestinal Stromal Tumour\u2013A Methodological Assessment Study.<\/strong> Brinch CM, Aggerholm-Pedersen N, Poulsen TS, Skovrider-Ruminski W, De Heer P, Penninga L, Krarup-Hansen A, Hogdall E. Anticancer Research. 2022 Nov 1;42(11):5527-37.
<\/strong><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

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Download<\/span><\/a><\/a><\/u><\/p>\n

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Cat Number: CKIT-RT44<\/strong><\/p>\n

The c-Kit Mutation Detection Kit is a PCR-based assay that detects somatic mutations in the c-KIT gene, which are associated with tumor development, prognosis, and response to targeted therapies in cancers such as GIST, melanoma, and mastocytosis.<\/p>\n

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