{"id":4358,"date":"2025-04-02T17:50:24","date_gmt":"2025-04-02T17:50:24","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4358"},"modified":"2026-04-22T23:05:07","modified_gmt":"2026-04-22T23:05:07","slug":"kras-mutation-analysis","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/kras-mutation-analysis\/","title":{"rendered":"KRAS Mutation Analysis"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” global_colors_info=”{}”][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The KRAS gene encodes a small GTPase that plays a crucial role in transmitting signals from the epidermal growth factor receptor (EGFR) to downstream pathways involved in cell proliferation and survival. Mutations in KRAS, particularly in codons 12, 13, 61, 117, and 146, are frequently found in cancers such as metastatic colorectal cancer (mCRC), lung adenocarcinoma, and thyroid cancer. Studies have shown that tumors harboring KRAS mutations are less likely to respond to anti-EGFR antibody therapy, making KRAS mutation testing a component of personalized treatment selection.<\/p>\n

The KRAS Mutation Analysis Kit is a real-time PCR-based assay designed for the specific and sensitive detection of somatic KRAS mutations. Using allele-specific PCR and fluorescent hydrolysis probes, the assay preferentially amplifies mutant DNA even in samples with a high proportion of wild-type DNA.<\/p>\n

An internal control gene ensures sample integrity and amplification efficiency.<\/p>\n

The KRAS Mutation Analysis Kit detects the following mutations in KRAS:<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Exon<\/strong><\/td>\nMutation<\/strong><\/td>\nNucleotide Change<\/strong><\/td>\nAmino Acid Change<\/strong><\/td>\nCOSMIC ID<\/strong><\/td>\n<\/tr>\n
2<\/td>\nG12D<\/td>\nc.35G>A<\/td>\nGlycine (G) to Aspartic acid (D)<\/td>\n521<\/td>\n<\/tr>\n
G12C<\/td>\nc.34G>T<\/td>\nGlycine (G) to Cysteine (C)<\/td>\n516<\/td>\n<\/tr>\n
G12S<\/td>\nc.34G>A<\/td>\nGlycine (G) to Serine (S)<\/td>\n517<\/td>\n<\/tr>\n
G12R<\/td>\nc.34G>C<\/td>\nGlycine (G) to Arginine (R)<\/td>\n518<\/td>\n<\/tr>\n
G12A<\/td>\nc.35G>C<\/td>\nGlycine (G) to Alanine (A)<\/td>\n522<\/td>\n<\/tr>\n
G12V<\/td>\nc.35G>T<\/td>\nGlycine (G) to Valine (V)<\/td>\n520<\/td>\n<\/tr>\n
G13D<\/td>\nc.38G>A<\/td>\nGlycine (G) to Aspartic acid (D)<\/td>\n532<\/td>\n<\/tr>\n
3<\/td>\nQ61H<\/td>\nc.183A>C & c.183A>T<\/td>\nGlutamine (Q) to Histidine (H)<\/td>\n554 & 555<\/td>\n<\/tr>\n
Q61L<\/td>\nc.182A>T<\/td>\nGlutamine (Q) to Leucine (L)<\/td>\n553<\/td>\n<\/tr>\n
Q61R<\/td>\nc.182A>G<\/td>\nGlutamine (Q) to Arginine (R)<\/td>\n552<\/td>\n<\/tr>\n
4<\/td>\nK117N<\/td>\nc.351A>C & c.351A>T<\/td>\nLysine (K) to Asparagine (N)<\/td>\n19940 & 28519<\/td>\n<\/tr>\n
K117R<\/td>\nc.350A>G<\/td>\nLysine (K) to Arginine (R)<\/td>\n1178068<\/td>\n<\/tr>\n
K117E<\/td>\nc.349A>G<\/td>\nLysine (K) to Glutamic acid (E)<\/td>\n1360831<\/td>\n<\/tr>\n
A146T<\/td>\nc.436G>A<\/td>\nAlanine (A) to Threonine (T)<\/td>\n19404<\/td>\n<\/tr>\n
A146P<\/td>\nc.436G>C<\/td>\nAlanine (A) to Proline (P)<\/td>\n19905<\/td>\n<\/tr>\n
A146V<\/td>\nc.437C>T<\/td>\nAlanine (A) to Valine (V)<\/td>\n19900<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n For More Information<\/span><\/a>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The KRAS Mutation Analysis Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.<\/p>\n

EntroGen\u02bcs KRAS mutation screening panel requires a real-time PCR instrument capable of detecting VIC and FAM fluorescent probes.<\/p>\n

This test includes reagents required for PCR amplification\/detection, as well as validated reaction controls.<\/p>\n

Columns and reagents for DNA isolation are not included.\u00a0Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA\u00ae Genomic DNA Isolation Kit<\/a>.<\/p>\n

We suggest using the <\/span>Internal Quality Control Assay (cat. no. IQCA<\/span><\/span>–<\/span><\/a>RT50)<\/a> to determine the optimal amount of input DNA prior to runnin<\/span>g this<\/span> assay.<\/span><\/span><\/p>\n

\"\"\"\" \u00a0\"\"\u00a0\u00a0\"\" \"\"<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n\n\n\n\n\n\n\n
\"\"<\/a><\/td>\nComparison of KRAS Mutation Tests in Colorectal Cancer Patients.<\/strong> Poster presented at the 20th National Pathology Congress, Turkey, September 2010. Veysel Sabri Han\u00e7er, G\u00f6khan Demir, \u0130lknur T\u00fcrkmen, Murat B\u00fcy\u00fckdo\u011fan, Nuray Ba\u015fs\u00fcll\u00fc, Tuncay Altu\u011f, Reyhan Diz-K\u00fc\u00e7\u00fckkaya, G\u00fclen B\u00fclb\u00fcl-Do\u011fusoy. Hancer VS, Buyukdogan M, T\u00fcrkmen I, Bassullu N, Altug T, Diz-Kucukkaya R, Bulbul-Dogusoy G, Demir G. Genetic Test Mol Biomarkers. 2011 Jun 23. Pubmed ID 21699410<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nKRAS and BRAF Mutation Analysis in Colorectal Adenocarcinoma Specimens with a Low Percentage of Tumor Cells.<\/strong> Lewandowska MA, J\u00f3\u017awicki W, Zurawski B. Mol Diagn Ther. 2013 Apr 20. Pubmed ID 23606169<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nAnalysis of KRAS and BRAF Genes Mutation in the Central Nervous System Metastases of Non-Small Cell Lung Cancer.<\/strong> Nico\u015b M1, Krawczyk P, Jarosz B, Sawicki M, Szumi\u0142\u0142o J, Trojanowski T, Milanowski J. Clin Exp Med. 2015 Apr 23. Pubmed ID 25902737<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nCetuximab in Treatment of Metastatic Colorectal Cancer: Final Survival Analyses and Extended RAS Data from the NORDIC-VII Study.<\/strong> Guren, Thomsen, Kure, Sorbye, Glimelius, Pfeiffer, \u00d6sterlund, Sigurdsson, Lothe, Dalsgaard, Skovlund, Christoffersen, Tveit. Comparison of KRAS Mutation Tests in Colorectal Cancer Patients. Br J Cancer. 2017 May 9;116(10):1271-1278. Pubmed ID 28399112<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nAnalysis of KRAS, NRAS, BRAF, and PIK3CA Mutations Could Predict Metastases in Colorectal Cancer: A Preliminary Study.<\/strong> Wojas-Krawczyk K, Kalinka-Warzocha E, Reszka K, Nico\u015b M, Szumi\u0142o J, Ma\u0144dziuk S, Szczepaniak K, Kupnicka D, Lewandowski R, Milanowski J, Krawczyk P. Adv Clin Exp Med. 2018 Aug 7. Pubmed ID 30085422<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n

 <\/p>\n

 <\/p>\n[\/et_pb_tab][\/et_pb_tabs][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_related_products include_categories=”44″ show_price=”off” show_rating=”off” show_sale_badge=”off” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_wc_related_products][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_section]\n","protected":false},"excerpt":{"rendered":"

Cat Number: KRAS-RT50<\/strong><\/p>\n

The KRAS Mutation Analysis Kit is a real-time PCR-based assay that detects somatic mutations in the KRAS gene, which are associated with tumor progression and resistance to EGFR-targeted therapies.<\/p>\n

 <\/p>\n