{"id":4376,"date":"2025-04-04T23:12:19","date_gmt":"2025-04-04T23:12:19","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4376"},"modified":"2025-06-23T19:25:28","modified_gmt":"2025-06-23T19:25:28","slug":"kras-braf-mutation-analysis-kit","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/kras-braf-mutation-analysis-kit\/","title":{"rendered":"KRAS\/BRAF Mutation Analysis Kit"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]\n

The KRAS and BRAF genes encode key proteins in the EGFR signaling pathway, which regulates cell proliferation and survival. Mutations in KRAS\u2014particularly in codons 12, 13, 61, 117, and 146\u2014and in BRAF, such as the V600E mutation, are frequently found in cancers like metastatic colorectal cancer (mCRC), lung adenocarcinoma, and thyroid cancer. These mutations are linked to reduced effectiveness of anti-EGFR antibody therapies, highlighting the importance of comprehensive KRAS and BRAF mutation testing for informing therapeutic strategies.<\/p>\n

The KRAS\/BRAF Mutation Analysis Kit is a real-time PCR-based assay designed for the rapid, specific, and sensitive detection of select KRAS and BRAF mutations.<\/p>\n

Utilizing allele-specific PCR and fluorescent hydrolysis probes, the assay preferentially amplifies mutant DNA\u2014even in the presence of excess wild-type DNA\u2014while an internal control ensures sample quality and amplification performance.<\/p>\n

The KRAS\/BRAF Mutation Analysis Kit detects the following mutations in KRAS:<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Exon<\/strong><\/td>\nMutation<\/strong><\/td>\nNucleotide Change<\/strong><\/td>\nAmino Acid Change<\/strong><\/td>\nCOSMIC ID<\/strong><\/td>\n<\/tr>\n
2<\/td>\nG12D<\/td>\nc.35G>A<\/td>\nGlycine (G) to Aspartic acid (D)<\/td>\n521<\/td>\n<\/tr>\n
G12C<\/td>\nc.34G>T<\/td>\nGlycine (G) to Cysteine (C)<\/td>\n516<\/td>\n<\/tr>\n
G12S<\/td>\nc.34G>A<\/td>\nGlycine (G) to Serine (S)<\/td>\n517<\/td>\n<\/tr>\n
G12R<\/td>\nc.34G>C<\/td>\nGlycine (G) to Arginine (R)<\/td>\n518<\/td>\n<\/tr>\n
G12A<\/td>\nc.35G>C<\/td>\nGlycine (G) to Alanine (A)<\/td>\n522<\/td>\n<\/tr>\n
G12V<\/td>\nc.35G>T<\/td>\nGlycine (G) to Valine (V)<\/td>\n520<\/td>\n<\/tr>\n
G13D<\/td>\nc.38G>A<\/td>\nGlycine (G) to Aspartic acid (D)<\/td>\n532<\/td>\n<\/tr>\n
3<\/td>\nQ61H<\/td>\nc.183A>C & c.183A>T<\/td>\nGlutamine (Q) to Histidine (H)<\/td>\n554 & 555<\/td>\n<\/tr>\n
Q61L<\/td>\nc.182A>T<\/td>\nGlutamine (Q) to Leucine (L)<\/td>\n553<\/td>\n<\/tr>\n
Q61R<\/td>\nc.182A>G<\/td>\nGlutamine (Q) to Arginine (R)<\/td>\n552<\/td>\n<\/tr>\n
4<\/td>\nK117N<\/td>\nc.351A>C & c.351A>T<\/td>\nLysine (K) to Asparagine (N)<\/td>\n19940 & 28519<\/td>\n<\/tr>\n
K117R<\/td>\nc.350A>G<\/td>\nLysine (K) to Arginine (R)<\/td>\n1178068<\/td>\n<\/tr>\n
K117E<\/td>\nc.349A>G<\/td>\nLysine (K) to Glutamic acid (E)<\/td>\n1360831<\/td>\n<\/tr>\n
A146T<\/td>\nc.436G>A<\/td>\nAlanine (A) to Threonine (T)<\/td>\n19404<\/td>\n<\/tr>\n
A146P<\/td>\nc.436G>C<\/td>\nAlanine (A) to Proline (P)<\/td>\n19905<\/td>\n<\/tr>\n
A146V<\/td>\nc.437C>T<\/td>\nAlanine (A) to Valine (V)<\/td>\n19900<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

and BRAF:<\/p>\n\n\n\n\n
Exon<\/strong><\/td>\nMutation<\/strong><\/td>\nNucleotide Change<\/strong><\/td>\nAmino Acid Change<\/strong><\/td>\nCOSMIC ID<\/strong><\/td>\n<\/tr>\n
15<\/td>\nV600E<\/td>\nc.1799T>A<\/td>\nValine (V) to Glutamic acid (E)<\/td>\n476<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n For More Information<\/span><\/a>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The KRAS\/BRAF Mutation Analysis Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.<\/p>\n

EntroGen\u02bcs KRAS\/BRAF mutation screening panel requires a real-time PCR instrument capable of detecting VIC and FAM fluorescent probes.<\/p>\n

This test includes reagents required for PCR amplification\/detection, as well as validated reaction controls.<\/p>\n

Columns and reagents for DNA isolation are not included. Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA\u00ae Genomic DNA Isolation Kit<\/a>.<\/p>\n

\"\"\"\" \"\" \u00a0\u00a0\"\" \"\"<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n\n\n\n\n\n\n\n
\"\"<\/a><\/td>\nComparison of KRAS Mutation Tests in Colorectal Cancer Patients.<\/strong> Poster presented at the 20th National Pathology Congress, Turkey, September 2010. Veysel Sabri Han\u00e7er, G\u00f6khan Demir, \u0130lknur T\u00fcrkmen, Murat B\u00fcy\u00fckdo\u011fan, Nuray Ba\u015fs\u00fcll\u00fc, Tuncay Altu\u011f, Reyhan Diz-K\u00fc\u00e7\u00fckkaya, G\u00fclen B\u00fclb\u00fcl-Do\u011fusoy. Hancer VS, Buyukdogan M, T\u00fcrkmen I, Bassullu N, Altug T, Diz-Kucukkaya R, Bulbul-Dogusoy G, Demir G. Genetic Test Mol Biomarkers. 2011 Jun 23. Pubmed ID 21699410<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nKRAS and BRAF Mutation Analysis in Colorectal Adenocarcinoma Specimens with a Low Percentage of Tumor Cells.<\/strong> Lewandowska MA, J\u00f3\u017awicki W, Zurawski B. Mol Diagn Ther. 2013 Apr 20. Pubmed ID 23606169<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nAnalysis of KRAS and BRAF Genes Mutation in the Central Nervous System Metastases of Non-Small Cell Lung Cancer.<\/strong> Nico\u015b M1, Krawczyk P, Jarosz B, Sawicki M, Szumi\u0142\u0142o J, Trojanowski T, Milanowski J. Clin Exp Med. 2015 Apr 23. Pubmed ID 25902737<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nCetuximab in Treatment of Metastatic Colorectal Cancer: Final Survival Analyses and Extended RAS Data from the NORDIC-VII Study.<\/strong> Guren, Thomsen, Kure, Sorbye, Glimelius, Pfeiffer, \u00d6sterlund, Sigurdsson, Lothe, Dalsgaard, Skovlund, Christoffersen, Tveit. Comparison of KRAS Mutation Tests in Colorectal Cancer Patients. Br J Cancer. 2017 May 9;116(10):1271-1278. Pubmed ID 28399112<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nAnalysis of KRAS, NRAS, BRAF, and PIK3CA Mutations Could Predict Metastases in Colorectal Cancer: A Preliminary Study.<\/strong> Wojas-Krawczyk K, Kalinka-Warzocha E, Reszka K, Nico\u015b M, Szumi\u0142o J, Ma\u0144dziuk S, Szczepaniak K, Kupnicka D, Lewandowski R, Milanowski J, Krawczyk P. Adv Clin Exp Med. 2018 Aug 7. Pubmed ID 30085422<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n[\/et_pb_tab][\/et_pb_tabs][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_related_products orderby=”menu_order” include_categories=”44″ show_price=”off” show_rating=”off” show_sale_badge=”off” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_wc_related_products][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_section]\n","protected":false},"excerpt":{"rendered":"

Cat Number: KRBR-RT50<\/strong><\/p>\n

The KRAS\/BRAF Mutation Analysis Kit is a rapid, real-time PCR assay that detects clinically relevant mutations to help identify mCRC patients unlikely to benefit from EGFR-targeted therapy, enabling more informed and personalized treatment decisions.<\/p>\n

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