{"id":4411,"date":"2025-04-07T20:58:12","date_gmt":"2025-04-07T20:58:12","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4411"},"modified":"2025-11-19T00:04:20","modified_gmt":"2025-11-19T00:04:20","slug":"ctdna-egfr-mutation-detection-kit","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/ctdna-egfr-mutation-detection-kit\/","title":{"rendered":"ctDNA EGFR Mutation Detection Kit"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The epidermal growth factor receptor (EGFR) is a membrane protein that plays a central role in transmitting signals from extracellular ligands to intracellular pathways, such as the Ras-Raf-MAPK cascade, which drive cell proliferation and survival. Mutations in EGFR, particularly in exons 18, 19, 20, and 21, are frequently found in lung cancer and other solid tumors and have been shown to predict response to EGFR-targeted therapies.<\/p>\n

The ctDNA EGFR Mutation Detection Kit is an ultra-sensitive, real-time PCR-based assay designed for the specific and sensitive detection of somatic EGFR mutations from circulating tumor DNA (ctDNA) isolated from plasma. Using mutant-specific primers and fluorescent hydrolysis probes, the assay preferentially amplifies mutant sequences even in samples with a high proportion of wild-type DNA.<\/p>\n

This highly reliable and efficient assay provides valuable insights into tumor genetics.<\/p>\n

A summary of mutations detected in each reaction is shown in the table below:<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Reaction<\/strong><\/td>\nMutation<\/strong><\/td>\n<\/tr>\n
1<\/td>\nExon 19 Deletions<\/td>\n<\/tr>\n
T790M<\/td>\n<\/tr>\n
L858R<\/td>\n<\/tr>\n
2<\/td>\nC797S<\/td>\n<\/tr>\n
3<\/td>\nL861Q<\/td>\n<\/tr>\n
4<\/td>\nS768I<\/td>\n<\/tr>\n
5<\/td>\nG719X<\/td>\n<\/tr>\n
6<\/td>\nEx20InsGGT\/CAC<\/td>\n<\/tr>\n
7<\/td>\nEx20Ins9 (c.2300_2308dup)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n For More Information<\/span><\/a>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The ctDNA EGFR Mutation Detection Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.<\/p>\n

EntroGen\u02bcs ctDNA EGFR Mutation Detection Kit requires a real-time PCR instrument capable of detecting VIC, FAM, ROX, and CY5 fluorescent probes.<\/p>\n

This test includes reagents required for PCR amplification\/detection, as well as validated reaction controls.<\/p>\n

Columns and reagents for cell-free DNA isolation are not included. Cell-free DNA can be extracted using EntroGen\u2019s Cell-Free DNA Isolation Kit<\/a>.<\/p>\n

\"\"\"\"\u00a0\"\"\u00a0 \u00a0\"\" \"\"<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]\n\n\n\n\n\n\n\n
\"\"<\/a><\/td>\nImplementation of a Real-Time PCR based plasma ctDNA analysis for detection of progressive disease acquired by EGFR T790M mediated TKI resistance in NSCLC patients<\/strong> \u2013 Jensen, S.G, et.al.<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nIs liquid biopsy a good molecular monitoring tool? Detection of L858R and T790M EGFR mutations in circulating tumor DNA derived from NSCLC patient treated with gefitinib (case study)<\/strong> \u2013 Katarzyna, E, Nalejska, E., Lewandowska, M., et. al.<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nAssessment of EGFR gene mutations in cf-DNA in monitoring of response to EGFR TKIs in patients with lung adenocarcinoma<\/strong> \u2013 Nico\u015b, Marcin, Wojas-Krawczyk, Kamila, et. al.<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nDetection of somatic mutations in ctDNA derived from adenocarcinoma patients \u2013 EGFR tyrosine kinase inhibitor monitoring preliminary study<\/strong> \u2013 Lewandowska, Marzena Anna, Nalejska, Ewelina, et. al.<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nThe efficacy of T790M mutation testing in liquid biopsy\u2014Real clinic data.<\/strong> Krawczyk P, Grzycka-Kowalczyk L, B\u0142ach J, Reszka K, Chmielewska I, Kieszko R, W\u00f3jcik-Superczy\u0144ska M, Szczyrek M, Jankowski T, Milanowski J.\u00a0 PLoS One. 2022 May 6;17(5):e0267846. doi: 10.1371\/journal.pone.0267846. PMID: 35522668; PMCID: PMC9075651.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n

 <\/p>\n[\/et_pb_tab][\/et_pb_tabs][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_related_products include_categories=”current” show_price=”off” show_rating=”off” show_sale_badge=”off” _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_wc_related_products][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_section]\n","protected":false},"excerpt":{"rendered":"

Cat Number: ctEGFR-48<\/strong><\/p>\n

The ctDNA EGFR Mutation Detection Kit is a real-time PCR-based assay that detects somatic EGFR mutations in exons 18, 19, 20, and 21 from circulating tumor DNA, providing actionable insights for targeted therapy in lung cancer when tumor tissue is unavailable.<\/p>\n

 <\/p>\n