{"id":4418,"date":"2025-04-08T16:06:39","date_gmt":"2025-04-08T16:06:39","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4418"},"modified":"2025-11-18T23:59:02","modified_gmt":"2025-11-18T23:59:02","slug":"egfr-mutation-analysis-kit","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/egfr-mutation-analysis-kit\/","title":{"rendered":"EGFR Mutation Analysis Kit"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n
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The epidermal growth factor receptor (EGFR) gene encodes a receptor tyrosine kinase that plays a central role in transmitting signals from extracellular ligands to downstream pathways involved in cell proliferation and survival. Mutations in EGFR, particularly in exons 18, 19, 20, and 21, are frequently found in various solid tumors.<\/p>\n

The EGFR Mutation Analysis Kit is a real-time PCR-based assay designed for the specific and sensitive detection of somatic EGFR mutations. Using mutant-specific primers and fluorescent hydrolysis probes, the assay preferentially amplifies mutant DNA even in samples with a high proportion of wild-type DNA.<\/p>\n

This highly reliable and efficient assay provides valuable insights into tumor genetics.<\/p>\n

EntroGen\u2019s EGFR Mutation Analysis Kit detects the following EGFR mutations:<\/p>\n

\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Exon 18<\/strong><\/td>\nExon 19<\/strong><\/td>\nExon 20<\/strong><\/td>\nExon 21<\/strong><\/td>\n<\/tr>\n
G719A \u2013 2156G>C<\/td>\n2235-2249 del 15<\/td>\nT790M \u2013 2369C>T<\/td>\nL858R \u2013 2573T>G<\/td>\n<\/tr>\n
G719S \u2013 2155G>A<\/td>\n2235-2252>AAT del 18<\/td>\nS768I \u2013 2303G>T<\/td>\nL861Q \u2013 2582T>A<\/td>\n<\/tr>\n
G719C \u2013 2155G>T<\/td>\n2236-2253 del 18<\/td>\n2307-2308 ins GCCAGCGTG<\/td>\n<\/tr>\n
G719D \u2013 2156G>A<\/td>\n2237-2251 del 15<\/td>\n2319-2320 ins CAC<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2237-2254 del 18<\/td>\n2310-2311 ins GGT<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2237-2255>T del 19<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2236-2250 del 15<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2238-2255 del 18<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2238-2248>GC del 11<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2238-2252>GCA del 15<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2233-2247 del 15<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2234-2248 del 15<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2235-2246 del 12<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2235-2248>AATTC<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2235-2251>AATTC<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2235-2252>AAT<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2235-2255>AAT<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2236-2248>AGAC<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2236-2248>CAAC<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2236-2256 del 21<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2237-2252>T<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2239-2247 del 9<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2239-2256 del 18<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2239-2248>C del 10<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2239-2258>CA del 20<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2240-2251 del 12<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2240-2257 del 18<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2240-2254 del 15<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2239-2251>C del 13<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2237-2253>TC<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2237-2253>TTCCT<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2237-2253>TTGCT<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2237-2256>TC<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2237-2257>TCT<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2238-2252 del 15<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2239-2252>CA<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2239-2256>CAA<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2239-2262 del 24<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2248-2273>CC<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2252-2275 del 24<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2252-2276>A<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2252-2277>AT<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2253-2276 del 24<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n2254-2277 del 24<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

\n For More Information<\/span><\/a>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]The EGFR Mutation Analysis Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.<\/p>\n

EntroGen\u02bcs EGFR Mutation Analysis Kit requires a real-time PCR instrument capable of detecting VIC and FAM fluorescent probes.<\/p>\n

This test includes reagents required for PCR amplification\/detection, as well as validated reaction controls.<\/p>\n

Columns and reagents for DNA isolation are not included.\u00a0Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA\u00ae Genomic DNA Isolation Kit<\/a>.<\/p>\n

We suggest using the <\/span>Internal Quality Control Assay (cat. no. IQCA<\/span><\/span>–<\/span><\/a>RT50)<\/a> to determine the optimal amount of input DNA prior to runnin<\/span>g this<\/span> assay.<\/span><\/span><\/p>\n

\"\"\"\"\u00a0\"\"\u00a0 \u00a0\u00a0\"\"\u00a0\"\"
\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]\n\n\n\n\n\n\n\n\n
\"\"<\/a><\/td>\nCytology smears as diagnostic material for EGFR gene testing in non-small cell lung cancer.\u00a0 <\/strong>Powr\u00f3zek T, Krawczyk P, Pankowski J, Reszka K, Jakubiak M, Obrochta A, Wojas-Krawczyk K, Buczkowski J, Milanowski J.\u00a0 Tumori. 2015 Nov 14;101(6):e151-3. Pubmed ID 26108238<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nThe Use of a Two-Tiered Testing Strategy for the Simultaneous Detection of Small EGFR Mutations and EGFR Amplification in Lung Cancer.<\/strong> Lewandowska MA, Czubak K, Klonowska K, Jozwicki W, Kowalewski J, Kozlowski P. PLoS One. 2015 Feb 26;10(2):e0117983. Pubmed ID 25719557<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nBorderline Brenner tumor of the ovary: a case report with immunohistochemical and molecular study.<\/strong> Rossella De Cecio, Monica Cantile, Francesca Collina, Laura Marra, Clemente Santonastaso, Cono Scaffa, Gerardo Botti, Nunzia Simona Losito. J Ovarian Res. 2014 Oct 29;7:101. Pubmed ID 25358264<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nDiscrepancies between ALK protein disruption and occurrence of ALK gene rearrangements in Polish NSCLC patients.<\/strong> Grenda A, Bo\u017cena J, Pawe\u0142 K, et. al.\u00a0J Thorac Dis. 2018 Aug; 10(8): 4994\u20135009.<\/span>\u00a0 Pubmed ID 30233874<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nThe efficacy of T790M mutation testing in liquid biopsy\u2014Real clinic data.<\/strong> Krawczyk P, Grzycka-Kowalczyk L, B\u0142ach J, Reszka K, Chmielewska I, Kieszko R, W\u00f3jcik-Superczy\u0144ska M, Szczyrek M, Jankowski T, Milanowski J.\u00a0 PLoS One. 2022 May 6;17(5):e0267846. doi: 10.1371\/journal.pone.0267846. PMID: 35522668; PMCID: PMC9075651.<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\n EPAS (EntroGen Variant Analysis Software) Data Analysis<\/strong><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n

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Cat Number: EGFR-RT52<\/strong><\/p>\n

The EGFR Mutation Analysis Kit is a highly sensitive real-time PCR assay that detects key EGFR mutations in exons 18\u201321, enabling accurate and efficient mutation profiling to guide targeted cancer therapy decisions.<\/p>\n

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