{"id":4426,"date":"2025-04-08T17:48:10","date_gmt":"2025-04-08T17:48:10","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4426"},"modified":"2025-06-23T19:27:53","modified_gmt":"2025-06-23T19:27:53","slug":"ctdna-ras-mutation-detection-kit","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/ctdna-ras-mutation-detection-kit\/","title":{"rendered":"ctDNA RAS Mutation Detection Kit"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” global_colors_info=”{}”][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The ctDNA RAS Mutation Detection Kit is a highly sensitive, real-time PCR-based assay designed for the detection of somatic mutations in KRAS<\/em> exon 2 and NRAS<\/em> exons 2 and 3 from circulating tumor DNA (ctDNA) extracted from plasma. Mutations in KRAS<\/em> (codons 12, 13) and NRAS<\/em> (codons 12, 13, and 61) are frequently associated with resistance to anti-EGFR therapies in cancers such as metastatic colorectal cancer, highlighting the importance of RAS mutation screening for informing personalized treatment selection.<\/p>\n

The assay utilizes allele-specific primers and fluorescent hydrolysis probes to preferentially amplify mutant DNA even in the presence of a high proportion of wild-type DNA. Two reactions detect the following mutations: KRAS G12\/G13, NRAS G12\/G13 (Reaction A), and NRAS Q61 (Reaction B).<\/p>\n

This minimally invasive, plasma-based assay enables detection of clinically relevant RAS mutations when tumor tissue is insufficient or unavailable, offering an important tool for guiding therapeutic decisions in cancer management.<\/p>\n

The list of KRAS and NRAS mutations detected by this kit is:<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene<\/strong><\/td>\nExon<\/strong><\/td>\nAA Change<\/strong><\/td>\nNucleotide Change<\/strong><\/td>\nCosmic ID<\/strong><\/td>\nDetected by Primer<\/strong><\/td>\n<\/tr>\n
KRAS<\/td>\n2<\/td>\nG12A<\/td>\nc.35G>C<\/td>\n522<\/td>\nA<\/td>\n<\/tr>\n
G12D<\/td>\nc.35G>A<\/td>\n521<\/td>\n<\/tr>\n
G12R<\/td>\nc.34G>C<\/td>\n518<\/td>\n<\/tr>\n
G12V<\/td>\nc.35G>T<\/td>\n520<\/td>\n<\/tr>\n
G12C<\/td>\nc.34G>T<\/td>\n516<\/td>\n<\/tr>\n
G12S<\/td>\nc.34G>A<\/td>\n517<\/td>\n<\/tr>\n
G13D<\/td>\nc.38G>A<\/td>\n532<\/td>\n<\/tr>\n
G13C<\/td>\nc.37G>T<\/td>\n527<\/td>\n<\/tr>\n
G13R<\/td>\nc.37G>C<\/td>\n529<\/td>\n<\/tr>\n
G13S<\/td>\nc.37G>A<\/td>\n528<\/td>\n<\/tr>\n
G13A<\/td>\nc.38G>C<\/td>\n533<\/td>\n<\/tr>\n
G13V<\/td>\nc.38G>T<\/td>\n534<\/td>\n<\/tr>\n
NRAS<\/td>\n2<\/td>\nG12D<\/td>\nc.35G>A<\/td>\n564<\/td>\nA<\/td>\n<\/tr>\n
G12C<\/td>\nc.34G>T<\/td>\n562<\/td>\n<\/tr>\n
G12S<\/td>\nc.34G>A<\/td>\n563<\/td>\n<\/tr>\n
G12A<\/td>\nc.35G>C<\/td>\n565<\/td>\n<\/tr>\n
G12R<\/td>\nc.34G>C<\/td>\n561<\/td>\n<\/tr>\n
G12V<\/td>\nc.35G>T<\/td>\n566<\/td>\n<\/tr>\n
G13R<\/td>\nc.37G>C<\/td>\n569<\/td>\n<\/tr>\n
G13V<\/td>\nc.38G>T<\/td>\n574<\/td>\n<\/tr>\n
3<\/td>\nQ61H (CAC)<\/td>\nc.183A>C<\/td>\n586<\/td>\nB<\/td>\n<\/tr>\n
Q61H (CAT)<\/td>\nc.183A>T<\/td>\n585<\/td>\n<\/tr>\n
Q61L<\/td>\nc.182A>T<\/td>\n583<\/td>\n<\/tr>\n
Q61K<\/td>\nc.181C>A<\/td>\n580<\/td>\n<\/tr>\n
Q61R<\/td>\nc.182A>G<\/td>\n584<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

For More Information<\/span><\/a><\/p>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The ctDNA RAS Mutation Detection Kit follows a simple and easy to use process. It can be completed in 2 hours and follows a simple straightforward process.<\/p>\n

EntroGen\u02bcs ctDNA RAS Mutation Detection Kit requires a real-time PCR instrument capable of detecting VIC, FAM, and CY5 fluorescent probes.<\/p>\n

This test includes reagents required for PCR amplification\/detection, as well as validated reaction controls.<\/p>\n

Columns and reagents for DNA isolation from plasma are not included. Cell-free DNA can be extracted using EntroGen\u2019s Cell-Free DNA Isolation Kit<\/a>.<\/p>\n

\"\"\"\" \u00a0\"\"\u00a0 \u00a0\"\" \"\"<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

 <\/p>\n\n\n\n\n\n\n
\"\"<\/a><\/td>\nKirsten ras mutations in patients with colorectal cancer: the \u2018RASCAL II\u2019 study.<\/strong> Andreyev, H.J., et al. (2001) Br J Cancer 85, 692-696<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nK-ras and p16 aberrations confer poor prognosis in human colorectal cancer.<\/strong> Esteller, M., et al. (2001)\u00a0 J Clin Oncol 19, 299-304<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nPanitumumab-FOLFOX4 Treatment and RAS Mutations in Colorectal Cancer.<\/strong> Douillard, JY. et al. (2013) N Engl J Med 369, 1023-1034<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nThe prognostic value of KRAS mutation by cell-free DNA in cancer patients: A systematic review and meta-analysis. <\/strong>Zhuang, R, Li S, Li Q, Guo X, Shen F, Sun H, et al. (2017)\u00a0 PLoS One 12(8): e0182562.<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n

 <\/p>\n

 <\/p>\n[\/et_pb_tab][\/et_pb_tabs][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_related_products include_categories=”current” show_price=”off” show_rating=”off” show_sale_badge=”off” _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_wc_related_products][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_section]\n","protected":false},"excerpt":{"rendered":"

Cat Number: CTRAS-RT40<\/strong><\/p>\n

The ctDNA RAS Mutation Detection Kit is a highly sensitive plasma-based assay for detecting clinically significant KRAS<\/em> and NRAS<\/em> mutations in circulating tumor DNA to guide personalized cancer treatment when tumor tissue is limited or unavailable.<\/p>\n

 <\/p>\n