{"id":4437,"date":"2025-04-09T22:57:07","date_gmt":"2025-04-09T22:57:07","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4437"},"modified":"2025-12-01T20:07:59","modified_gmt":"2025-12-01T20:07:59","slug":"mgmt-methylation-detection-kit-w-bisulfite-conversion-kit","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/mgmt-methylation-detection-kit-w-bisulfite-conversion-kit\/","title":{"rendered":"MGMT Methylation Detection Kit w\/ Bisulfite Conversion Kit"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” global_colors_info=”{}”][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The MGMT Methylation Detection Kit with Bisulfite Conversion Kit is a semi-quantitative, multiplexed, real-time PCR-based assay that detects methylation in the MGMT DMR2 region using bisulfite-treated DNA.<\/p>\n

The assay includes specific primers and fluorescent probes that selectively amplify and detect methylated cytosines, alongside an internal control gene (Actin B) for normalization and quality control.<\/p>\n

This comprehensive kit provides all necessary reagents for efficient bisulfite conversion and methylation detection.<\/p>\n

The MGMT Methylation Detection Kit utilizes the 5% Methylation Reference Control (MGMT-LPC) as a benchmark during the bisulfite conversion process. This control standard allows for the accurate calculation of process-corrected % methylation in unknown samples by accounting for run-to-run variations of the bisulfite reaction efficiency.<\/p>\n

Researchers and clinical labs can reliably assess MGMT methylation status from FFPE or fresh\/frozen tumor samples to support treatment decisions and prognostic evaluation.<\/p>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The MGMT Methylation Detection Kit with Bisulfite Conversion offers a straightforward, streamlined workflow that can be completed in under a day, with minimal hands-on time and easy-to-follow steps.<\/p>\n

EntroGen\u2019s MGMT Methylation Detection Kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes.<\/p>\n

This test includes all necessary reagents for both bisulfite conversion and real-time PCR amplification\/detection, along with validated controls. DNA is first extracted from FFPE or fresh\/frozen samples, then subjected to bisulfite treatment using the included reagents to convert unmethylated cytosines into uracil. The treated DNA is then amplified using methylation-specific primers and fluorescent probes that target the MGMT DMR2 region and an internal control gene.<\/p>\n

Columns and reagents for initial DNA extraction are not included in the kit. Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA\u00ae Genomic DNA Isolation Kit<\/a>.<\/p>\n

\"\"\"\" \"\" \"\"<\/span>\"\"\"\"\"\"<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n\n\n\n\n
\"\"<\/a><\/td>\nValidation of the EntroGen MGMT Promoter Methylation Detection Kit<\/a><\/td>\n<\/tr>\n
\"\"<\/a><\/td>\nWebinar Recording \u2013 MGMT Methylation Status \u2013 Impact of Proper Methylation Classification on GBM Treatment<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n[\/et_pb_tab][\/et_pb_tabs][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_related_products include_categories=”current” show_price=”off” show_rating=”off” show_sale_badge=”off” _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_wc_related_products][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_section]\n","protected":false},"excerpt":{"rendered":"

Cat Number: MGMTX-RT44<\/strong><\/p>\n

The MGMT Methylation Detection Kit is a real-time PCR assay that detects promoter methylation in the DMR2 region of the MGMT gene using bisulfite-converted DNA, providing a sensitive method for assessing epigenetic silencing associated with treatment response in glioblastoma and other tumors.<\/p>\n

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