{"id":4471,"date":"2025-04-15T22:08:30","date_gmt":"2025-04-15T22:08:30","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4471"},"modified":"2025-11-19T00:19:08","modified_gmt":"2025-11-19T00:19:08","slug":"ngs-targeted-hotspot-panel-2","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/ngs-targeted-hotspot-panel-2\/","title":{"rendered":"NGS Targeted Hotspot Panel (For Illumina\u00ae Platforms)"},"content":{"rendered":"[et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_title _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_title][et_pb_wc_price _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_price][et_pb_wc_description _builder_version=”4.27.4″ global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_add_to_cart _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_add_to_cart][et_pb_wc_meta _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_meta][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_tabs _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The NGS Targeted Hotspot Panel is a next-generation sequencing (NGS) assay designed to detect clinically relevant somatic mutations and copy number variations (CNVs) in solid tumors.<\/p>\n

The NGS Targeted Hotspot Panel employs a highly multiplexed, tiled amplicon-based enrichment strategy to ensure efficient and comprehensive coverage of critical hotspots. The assay targets 200 amplicons with an average size of 164 base pairs, optimized for high-performance sequencing. Indexed PCR and adapter ligation prepare libraries for compatibility with Illumina\u00ae sequencing platforms.<\/p>\n

The kit includes all necessary reagents for target enrichment and library preparation, and is validated for use with DNA derived from a variety of sample types including FFPE tissues, blood, fresh or frozen specimens, and cell lines. EntroGen\u2019s Variant Analysis (EVA) software complements the panel by enabling intuitive reporting of single nucleotide variants (SNVs), small insertions\/deletions (indels), and focal gene amplifications.<\/p>\n

For laboratories conducting comprehensive tumor profiling, the NGS Targeted Hotspot Panel offers a robust and streamlined solution to support personalized cancer research and precision oncology applications.<\/p>\n

The panel targets hotspot regions in 16 cancer-associated genes:<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene<\/strong><\/td>\nDisease<\/strong><\/td>\nCoverage<\/strong><\/td>\n<\/tr>\n
BRAF<\/td>\nMelanoma, Colorectal, Lung<\/td>\nExons 11 & 15<\/td>\n<\/tr>\n
EGFR<\/td>\nLung, Glioma<\/td>\nExons 3, 7, 12, 15, 18, 19, 20, 21<\/td>\n<\/tr>\n
ERBB2<\/td>\nBreast, Lung<\/td>\nExons 8, 14, 17, 18, 19, 20, 21, 24, 26<\/td>\n<\/tr>\n
HRAS<\/td>\nThyroid<\/td>\nExons 2, 3<\/td>\n<\/tr>\n
C-KIT<\/td>\nGIST, Melanoma<\/td>\nExons 2, 8, 9, 10, 11, 13, 14, 15, 17, 18<\/td>\n<\/tr>\n
IDH1<\/td>\nGlioma, AML<\/td>\nExon 2<\/td>\n<\/tr>\n
IDH2<\/td>\nGlioma, AML<\/td>\nExon 4<\/td>\n<\/tr>\n
JAK2<\/td>\nBone Marrow Disorders<\/td>\nExon 12, 14<\/td>\n<\/tr>\n
KRAS<\/td>\nColorectal, Lung<\/td>\nExons 2, 3, 4<\/td>\n<\/tr>\n
MET<\/td>\nLung<\/td>\nExons 2, 11, 14, 16, 18, 19, 20<\/td>\n<\/tr>\n
NRAS<\/td>\nColorectal, Melanoma<\/td>\nExons 2, 3, 4<\/td>\n<\/tr>\n
PDGFRA<\/td>\nGIST<\/td>\nExons 12, 14, 15, 18<\/td>\n<\/tr>\n
PIK3CA<\/td>\nColorectal, Breast, Ovarian<\/td>\nExons 1, 4, 6, 7, 9, 13,18, 20<\/td>\n<\/tr>\n
RET<\/td>\nThyroid<\/td>\nExons 10, 11, 13, 15, 16<\/td>\n<\/tr>\n
TERT<\/td>\nCNS, Bladder, Thyroid, Skin<\/td>\nPromoter<\/td>\n<\/tr>\n
TP53<\/td>\nBreast, Ovarian, Skin, Lung, Colorectal<\/td>\nEntire Exome<\/td>\n<\/tr>\n
PTEN*<\/td>\nEndometrial, Glioma, Prostate, Breast, Colon<\/td>\nExons 1-9<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

*Available upon request<\/p>\n

 <\/p>\n For More Information<\/span><\/a>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

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The NGS Targeted Hotspot Panel provides a streamlined and robust next-generation sequencing (NGS) workflow for the detection of somatic mutations and copy number variations in cancer-associated genes. The assay is compatible with DNA extracted from blood, fresh frozen tissues, cell lines, and high-quality FFPE samples, and is optimized for use on Illumina\u00ae sequencing platforms. Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA\u00ae Genomic DNA Isolation Kit<\/a>.<\/p>\n

This kit includes reagents required for target enrichment, library preparation, and indexing to ensure accurate and efficient sequencing. The workflow involves DNA quantification, highly multiplexed PCR-based target enrichment, and adaptor ligation, followed by purification and generation of NGS-ready libraries.<\/p>\n

Reagents and columns for DNA isolation are not included. To ensure optimal assay performance, DNA input should be quantified using a qPCR-based method prior to library preparation.<\/p>\n

The following suplimentary products are available, but not supplied:<\/p>\n