{"id":4473,"date":"2025-04-16T17:09:54","date_gmt":"2025-04-16T17:09:54","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4473"},"modified":"2025-07-02T18:32:20","modified_gmt":"2025-07-02T18:32:20","slug":"rna-fusion-gene-panel-2","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/rna-fusion-gene-panel-2\/","title":{"rendered":"RNA Fusion Gene Panel (For Illumina\u00ae Platforms)"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]\n

The RNA Fusion Gene Panel is a next-generation sequencing (NGS) assay designed to detect oncogenic gene fusion events in a broad spectrum of solid tumors and hematologic malignancies.<\/p>\n

These fusion events are frequently observed across multiple tumor types and play critical roles in cancer development by driving constitutive activation of signaling pathways such as MAPK and PI3K.<\/p>\n

The RNA Fusion Gene Panel utilizes a highly multiplexed, PCR-based target enrichment strategy to amplify 305 known fusion transcripts with an average amplicon size of 145 base pairs. The assay includes reagents for reverse transcription, indexed PCR, and adapter ligation, generating libraries compatible with Illumina\u00ae sequencing platforms.<\/p>\n

The kit provides all necessary components for fusion gene detection and library preparation, and is validated for use with RNA derived from a variety of sources including FFPE tissues, blood, fresh or frozen samples, and cultured cells. EntroGen\u2019s Variant Analysis (EVA) software supports data interpretation with a user-friendly interface that reports fusion gene identities along with genomic breakpoint positions.<\/p>\n

For laboratories focused on transcriptome-level tumor profiling, the RNA Fusion Gene Panel offers a robust and efficient tool for the sensitive detection of fusion events relevant to precision oncology research.<\/p>\n

The panel targets fusion transcripts involving 23 cancer-associated genes:<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Gene<\/strong><\/td>\nNumber of Detected Fusions<\/strong><\/td>\n<\/tr>\n
ABL<\/td>\n1<\/td>\n<\/tr>\n
ALK<\/td>\n61<\/td>\n<\/tr>\n
AKT3<\/td>\n1<\/td>\n<\/tr>\n
AXL<\/td>\n1<\/td>\n<\/tr>\n
BRAF<\/td>\n15<\/td>\n<\/tr>\n
EGFR<\/td>\n2<\/td>\n<\/tr>\n
ERBB2<\/td>\n1<\/td>\n<\/tr>\n
ERG<\/td>\n16<\/td>\n<\/tr>\n
ETV1<\/td>\n4<\/td>\n<\/tr>\n
ETV4<\/td>\n1<\/td>\n<\/tr>\n
ETV5<\/td>\n3<\/td>\n<\/tr>\n
FGFR1<\/td>\n4<\/td>\n<\/tr>\n
FGFR2<\/td>\n10<\/td>\n<\/tr>\n
FGFR3<\/td>\n25<\/td>\n<\/tr>\n
MET<\/td>\n10<\/td>\n<\/tr>\n
NTRK1<\/td>\n34<\/td>\n<\/tr>\n
NTRK2<\/td>\n18<\/td>\n<\/tr>\n
NTRK3<\/td>\n16<\/td>\n<\/tr>\n
PDGFRA<\/td>\n1<\/td>\n<\/tr>\n
PPARG<\/td>\n4<\/td>\n<\/tr>\n
RAF1<\/td>\n2<\/td>\n<\/tr>\n
RET<\/td>\n41<\/td>\n<\/tr>\n
ROS1<\/td>\n34<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n For More Information<\/span><\/a>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The RNA Fusion Gene Panel provides a streamlined and efficient next-generation sequencing (NGS) workflow for the detection of oncogenic gene fusion events in cancer-associated transcripts. The assay is compatible with RNA extracted from blood, fresh frozen tissues, and high-quality FFPE samples, and is optimized for use on Illumina\u00ae sequencing platforms.<\/p>\n

This kit includes reagents required for reverse transcription, target enrichment, library preparation, and indexing to ensure accurate and sensitive detection of fusion transcripts. The workflow involves RNA quantification, cDNA synthesis through reverse transcription, highly multiplexed PCR-based enrichment of fusion targets, and adaptor ligation, followed by purification and generation of NGS-ready libraries.<\/p>\n

Reagents and columns for RNA isolation are not included. To ensure optimal assay performance, RNA input should be quantified using a fluorometric or qPCR-based method prior to reverse transcription and library preparation.<\/p>\n

The Library Quantification Kit for Illumina\u00ae (EntroGen, catalog no. LIBQ-NGS)<\/a> is recommended, but not included with this kit.<\/p>\n

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\u00a0\u00a0\u00a0\"\"\"\"\u00a0<\/span>\"\"\"\"\u00a0\u00a0<\/span>\"\"\"\"\u00a0<\/span>\"\"\"\"\"\"<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n\n\n\n
\"\"<\/a><\/td>\nWebinar Recording:\u00a0 RNA Based NGS & RT-PCR Alternatives for Fusion Gene Detection in Solid Tumors<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n

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Cat Number: RFGP-NGS<\/strong><\/p>\n

The RNA Fusion Gene Panel is a multiplexed next-generation sequencing assay designed to detect key oncogenic gene fusions across 23 clinically relevant cancer genes for comprehensive transcript-level profiling.<\/p>\n

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