{"id":4753,"date":"2025-05-20T21:00:00","date_gmt":"2025-05-20T21:00:00","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=4753"},"modified":"2025-07-18T17:18:07","modified_gmt":"2025-07-18T17:18:07","slug":"purena-genomic-dna-isolation-kit","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/purena-genomic-dna-isolation-kit\/","title":{"rendered":"pureNA \u00ae Genomic DNA Isolation Kit"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” global_colors_info=”{}”][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The pureNA\u00ae Genomic DNA Isolation Kit is an efficient solution for extracting high-quality genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. Optimized for ease of use and scalability, DNA extracted using this kit is suitable for down-stream applications such as qPCR and next-generation sequencing (NGS).<\/p>\n

In performance evaluations, EntroGen\u2019s pureNA\u00ae Genomic DNA Isolation Kit demonstrated superior gDNA yields compared to a leading competitor. Genomic DNA was isolated from colorectal cancer (CRC) FFPE specimens across five cases, and yields were measured using both Qubit and EntroGen\u2019s qPCR-based DNA quantification assay (Cat. No. FQA-RT40). The results highlight the kit\u2019s ability to deliver consistent, higher-quality DNA for sensitive molecular analyses.<\/p>\n

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Figure 1:<\/strong> Total DNA yield determined by Qubit.<\/p>\n

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Figure 2:<\/strong> Amplifiable 150 bp DNA fragments determined by qPCR fragmentation quantification assay.<\/p>\n[\/et_pb_tab][et_pb_tab title=”Workflow” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

The pureNA\u00ae Genomic DNA Isolation Kit offers a straightforward and efficient workflow for extracting high-quality genomic DNA from FFPE tissue specimens. The process is optimized to remove paraffin, reverse formalin cross-links, and release DNA using heat, proteinase K digestion, and magnetic bead-based purification.<\/p>\n

The protocol is compatible with high-throughput processing.\u00a0 DNA is bound to magnetic beads, washed to remove impurities, and eluted in a ready-to-use buffer for downstream applications like qPCR and NGS.<\/p>\n

All necessary reagents for gDNA isolation from FFPE samples are provided with the exception of 100% ethanol, which is not included.<\/p>\n

This protocol also requires a magnetic stand for molecular applications compatible with 1.5ml microcentrifuge tubes for molecular applications.<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n

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Cat Number: GDNA-50<\/strong><\/p>\n

The pureNA\u00ae Genomic DNA Isolation Kit enables efficient, high-purity DNA extraction from FFPE tissues for downstream applications like qPCR.<\/p>\n

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