{"id":4761,"date":"2025-05-21T18:13:15","date_gmt":"2025-05-21T18:13:15","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&#038;p=4761"},"modified":"2026-02-09T17:44:54","modified_gmt":"2026-02-09T17:44:54","slug":"dna-fragmentation-quantification-assay","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/dna-fragmentation-quantification-assay\/","title":{"rendered":"DNA Fragmentation Quantification Assay"},"content":{"rendered":"[et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;Section&#8221; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; custom_padding=&#8221;6px||4px|||&#8221; collapsed=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.16&#8243; background_size=&#8221;initial&#8221; background_position=&#8221;top_left&#8221; background_repeat=&#8221;repeat&#8221; width=&#8221;100%&#8221; custom_padding=&#8221;0px||0px||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; custom_padding=&#8221;|||&#8221; global_colors_info=&#8221;{}&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_wc_breadcrumb admin_label=&#8221;Woo Breadcrumbs&#8221; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; specialty=&#8221;on&#8221; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; custom_padding=&#8221;0px||9px|||&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.16&#8243; custom_padding=&#8221;|||&#8221; global_colors_info=&#8221;{}&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_wc_images _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;|||-98px||&#8221; custom_padding=&#8221;|||23px||&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=&#8221;2_3&#8243; specialty_columns=&#8221;2&#8243; _builder_version=&#8221;4.16&#8243; custom_padding=&#8221;|||&#8221; global_colors_info=&#8221;{}&#8221; custom_padding__hover=&#8221;|||&#8221;][et_pb_row_inner column_structure=&#8221;1_2,1_2&#8243; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; custom_padding=&#8221;|0px||||&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column_inner type=&#8221;1_2&#8243; saved_specialty_column_type=&#8221;2_3&#8243; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_wc_title _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; header_font_size=&#8221;27px&#8221; custom_margin=&#8221;|-164px||||&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=&#8221;1_2&#8243; saved_specialty_column_type=&#8221;2_3&#8243; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column_inner saved_specialty_column_type=&#8221;2_3&#8243; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_wc_description admin_label=&#8221;Woo Product Short Description&#8221; _builder_version=&#8221;4.27.4&#8243; custom_margin=&#8221;-8px|||||&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_wc_description][et_pb_wc_meta show_sku=&#8221;off&#8221; admin_label=&#8221;Woo Product Meta&#8221; _builder_version=&#8221;4.27.2&#8243; global_colors_info=&#8221;{}&#8221;][\/et_pb_wc_meta][et_pb_button button_url=&#8221;https:\/\/entrogen.com\/web4\/product-information-request-form\/&#8221; button_text=&#8221;Request More Information&#8221; button_alignment=&#8221;left&#8221; admin_label=&#8221;Button&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; custom_button=&#8221;on&#8221; button_text_size=&#8221;13px&#8221; button_text_color=&#8221;#FFFFFF&#8221; button_bg_color=&#8221;#476e8d&#8221; button_border_color=&#8221;RGBA(255,255,255,0)&#8221; background_layout=&#8221;dark&#8221; custom_margin=&#8221;30px|||||&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; custom_padding=&#8221;0px||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; custom_padding=&#8221;6px|||||&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_tabs admin_label=&#8221;Tabs&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;24px|||||&#8221; custom_padding=&#8221;|30px|60px|||&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_tab title=&#8221;Product Overview&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]\n<p data-start=\"171\" data-end=\"534\">The DNA Fragmentation Quantification Assay is a qPCR-based assay designed to evaluate the quality and amplifiability of DNA extracted from FFPE and fresh\/frozen tissue samples. By amplifying three different fragment sizes (37 bp, 150 bp, and 300 bp) from a reference gene on chromosome 5, the assay provides a comprehensive assessment of DNA integrity.<\/p>\n<p data-start=\"536\" data-end=\"946\">Each sample is tested against a standard curve, yielding three key outputs:<\/p>\n<ul>\n<li data-start=\"536\" data-end=\"946\">DNA concentration (ng\/\u03bcl)<\/li>\n<li data-start=\"536\" data-end=\"946\">Amplifiable copy numbers for each fragment size<\/li>\n<li data-start=\"536\" data-end=\"946\">A calculated fragmentation ratio (F-ratio).<\/li>\n<\/ul>\n<p data-start=\"536\" data-end=\"946\">These values allow researchers to assess DNA suitability for downstream PCR-based applications and to normalize input amounts more accurately than traditional quantification methods.<\/p>\n[\/et_pb_tab][et_pb_tab title=&#8221;Workflow&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]\n<p>&nbsp;<\/p>\n<p data-start=\"154\" data-end=\"506\">The DNA Fragmentation Quantification Assay is a simple and efficient qPCR-based assay used to assess the integrity and amplifiability of FFPE-derived DNA. It evaluates fragmentation by amplifying three target sizes\u201437 bp, 150 bp, and 300 bp\u2014from a reference gene using SYBR\u00ae Green detection chemistry. Results are available in under 2 hours.<\/p>\n<p data-start=\"508\" data-end=\"777\">EntroGen\u2019s DNA Fragmentation Quantification Assay includes primer mixes, standards, and reagents needed for PCR amplification, along with analysis templates for automated interpretation.<\/p>\n<p data-start=\"779\" data-end=\"835\" data-is-last-node=\"\" data-is-only-node=\"\">Reagents and columns for DNA isolation are not included.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/05\/FQA-RT50-load-samples-267x300.png\" width=\"267\" height=\"300\" alt=\"\" class=\"wp-image-4763 alignnone size-medium\" \/><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/03\/Arrow-right-sized-74x300.png\" width=\"74\" height=\"300\" alt=\"\" class=\"wp-image-4164 alignnone size-medium\" srcset=\"https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/03\/Arrow-right-sized-74x300.png 74w, https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/03\/Arrow-right-sized.png 150w\" sizes=\"(max-width: 74px) 100vw, 74px\" \/><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/05\/FQA-RT50-run-in-pcr-267x300.png\" width=\"267\" height=\"300\" alt=\"\" class=\"wp-image-4764 alignnone size-medium\" \/> <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/03\/Arrow-right-sized-74x300.png\" width=\"74\" height=\"300\" alt=\"\" class=\"wp-image-4164 alignnone size-medium\" srcset=\"https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/03\/Arrow-right-sized-74x300.png 74w, https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/03\/Arrow-right-sized.png 150w\" sizes=\"(max-width: 74px) 100vw, 74px\" \/><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/05\/FQA-RT50-Analyze-Results-267x300.png\" width=\"267\" height=\"300\" alt=\"\" class=\"wp-image-4762 alignnone size-medium\" \/><\/p>\n[\/et_pb_tab][et_pb_tab title=&#8221;Resources&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]\n<p>Click to download lot-specific quality control data for your product:<\/p>\n<p><u><a href=\"https:\/\/entrogen.com\/coa\"><a href=\"https:\/\/entrogen.com\/coa\" class=\"su-button su-button-style-default\" style=\"color:#FFFFFF;background-color:#2D89EF;border-color:#246ec0;border-radius:5px\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"color:#FFFFFF;padding:0px 16px;font-size:13px;line-height:26px;border-color:#6cadf4;border-radius:5px;text-shadow:none\"> Download<\/span><\/a><\/a><\/u><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n[\/et_pb_tab][\/et_pb_tabs][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_wc_related_products include_categories=&#8221;current&#8221; show_price=&#8221;off&#8221; show_rating=&#8221;off&#8221; show_sale_badge=&#8221;off&#8221; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_wc_related_products][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_section]\n","protected":false},"excerpt":{"rendered":"<p>Cat Number: <strong>FQA-RT40<\/strong><\/p>\n<p>The DNA Fragmentation Quantification Assay is used to assess the quality and quantity of amplifiable DNA available for downstream applications like qPCR.<\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li>Assesses sample quality by measuring fragmentation ratio.<\/li>\n<li>Quantifies amount of amplifiable DNA for downstream qPCR and NGS assays.<\/li>\n<li>Predicts optimal DNA input for maximal and reliable data output in qPCR and NGS.<\/li>\n<li>Simple set up and interpretation.<\/li>\n<li>Automated analysis available<\/li>\n<li>For research use (RUO) in the U.S<\/li>\n<li>For in vitro diagnostic use (IVD) in the European Union<\/li>\n<\/ul>\n","protected":false},"featured_media":4918,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"[su_tabs][su_tab title=\"Overview\" disabled=\"no\" anchor=\"\" url=\"\" target=\"\" class=\"\"]\r\n\r\nMutations in the KRAS gene, commonly found in metastatic colorectal cancer (mCRC), lung adenocarcinoma, and thyroid cancer, can make tumors resistant to anti-EGFR treatments. Studies show that mutations in exons 2, 3, and 4 of the KRAS and NRAS genes reduce the effectiveness of these therapies, leading experts, including the American Society of Clinical Oncology (ASCO), to recommend genetic screening before treatment.\r\n\r\nThe RAS mutation analysis real-time assay utilizes allele-specific PCR, ensuring accurate detection using fluorescent hydrolysis probes. The panel is a qualitative test that detects somatic mutations found in exons 2, 3, and 4 of KRAS and NRAS genes. Below is a list of mutations detected by this kit.\r\n\r\nThe RAS Mutation Screening Panel identifies the presence of the mutations in a total of 8 reactions per sample. The test does not distinguish between mutations within each group.\r\n\r\n*A complementary add-on reaction that distinguishes KRAS G12C from other codon 12 mutations is available, upon request.\r\n\r\n<sup><img class=\"alignnone size-medium wp-image-4077\" src=\"http:\/\/entrogen.com\/web4\/wp-content\/uploads\/2024\/11\/KRAS-Mutation-Chart-300x128.png\" alt=\"\" width=\"300\" height=\"128\" \/>\r\n<\/sup><sup>1<\/sup> My Cancer Genome; www.mycancergenome.org (Colorectal Carcinoma, Colorectal Adenocarcinoma)\r\n\r\n[\/su_tab]\r\n[su_tab title=\"Workflow\" disabled=\"no\" anchor=\"\" url=\"\" target=\"blank\" class=\"\"]\r\n\r\nProduct Workflow\r\n\r\n[\/su_tab]\r\n[su_tab title=\"Resources\" disabled=\"no\" anchor=\"\" url=\"\" target=\"blank\" class=\"\"]\r\n\r\n&nbsp;\r\n\r\n&nbsp;\r\n\r\n<a href=\"http:\/\/entrogen.com\/web4\/videos\/ras.htm\">http:\/\/entrogen.com\/web4\/videos\/ras.htm<\/a>\r\n\r\n[\/su_tab][\/su_tabs]","_et_gb_content_width":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false},"product_brand":[],"product_cat":[39],"product_tag":[],"class_list":{"0":"post-4761","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-uncategorized","8":"first","9":"instock","10":"shipping-taxable","11":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product\/4761","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/media\/4918"}],"wp:attachment":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/media?parent=4761"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product_brand?post=4761"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product_cat?post=4761"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product_tag?post=4761"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}