{"id":4766,"date":"2025-05-21T21:36:54","date_gmt":"2025-05-21T21:36:54","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&#038;p=4766"},"modified":"2025-06-13T16:29:27","modified_gmt":"2025-06-13T16:29:27","slug":"internal-quality-control-assay","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/internal-quality-control-assay\/","title":{"rendered":"Internal Quality Control Assay"},"content":{"rendered":"[et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;Section&#8221; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; custom_padding=&#8221;6px||4px|||&#8221; collapsed=&#8221;off&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.16&#8243; background_size=&#8221;initial&#8221; background_position=&#8221;top_left&#8221; background_repeat=&#8221;repeat&#8221; width=&#8221;100%&#8221; custom_padding=&#8221;0px||0px||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; 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_module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column_inner saved_specialty_column_type=&#8221;2_3&#8243; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_wc_description admin_label=&#8221;Woo Product Short Description&#8221; _builder_version=&#8221;4.27.4&#8243; custom_margin=&#8221;-8px|||||&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_wc_description][et_pb_wc_meta show_sku=&#8221;off&#8221; admin_label=&#8221;Woo Product Meta&#8221; _builder_version=&#8221;4.27.2&#8243; global_colors_info=&#8221;{}&#8221;][\/et_pb_wc_meta][et_pb_button button_url=&#8221;https:\/\/entrogen.com\/web4\/product-information-request-form\/&#8221; button_text=&#8221;Request More Information&#8221; button_alignment=&#8221;left&#8221; admin_label=&#8221;Button&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; custom_button=&#8221;on&#8221; button_text_size=&#8221;13px&#8221; button_text_color=&#8221;#FFFFFF&#8221; button_bg_color=&#8221;#476e8d&#8221; button_border_color=&#8221;RGBA(255,255,255,0)&#8221; background_layout=&#8221;dark&#8221; custom_margin=&#8221;30px|||||&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; custom_padding=&#8221;0px||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; custom_padding=&#8221;6px|||||&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_tabs admin_label=&#8221;Tabs&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;24px|||||&#8221; custom_padding=&#8221;|30px|60px|||&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_tab title=&#8221;Product Overview&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]\n<p>The Internal Quality Control Assay is a real-time PCR-based test designed to confirm the presence of amplifiable human DNA in FFPE tissue samples prior to molecular testing. By targeting a conserved human genomic region, the assay ensures that extracted DNA is of sufficient quality and quantity for reliable downstream applications such as qPCR and next-generation sequencing (NGS).<\/p>\n<p>This quality check is especially critical for FFPE specimens, which are often compromised by formalin fixation and long-term storage.<\/p>\n<p>The assay helps prevent false-negative results by verifying specimen integrity before further analysis.<\/p>\n[\/et_pb_tab][et_pb_tab title=&#8221;Workflow&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]\n<p>The IQCA kit requires a real-time PCR instrument capable of detecting the VIC fluorophore.<\/p>\n<p>The kit includes:<\/p>\n<ul>\n<li>2x Reaction Master Mix<\/li>\n<li>Primer-probe mix for the detection of the internal control gene<\/li>\n<\/ul>\n<p>The kit does not include reagents and materials for DNA isolation.<\/p>\n<p>The reagents in the IQCA kit are sufficient for 50 reactions.<\/p>\n<p>After DNA isolation, set up reactions using reagents provided in the IQCA kit and run the assay on a compatible real-time PCR instrument. The assay may be completed in approximately 1 hour and 15 minutes from assay set up to results. Automated analysis is available to assist with data interpretation for certain instruments.<\/p>\n<a href=\"https:\/\/entrogen.com\/web4\/product-information-request-form\/\" class=\"su-button su-button-style-default\" style=\"color:#FFFFFF;background-color:#2D89EF;border-color:#246ec0;border-radius:5px\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"color:#FFFFFF;padding:0px 16px;font-size:13px;line-height:26px;border-color:#6cadf4;border-radius:5px;text-shadow:none\"> For More Information<\/span><\/a>\n[\/et_pb_tab][et_pb_tab title=&#8221;Resources&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]\n<table style=\"width: 100%; border-collapse: collapse; border-style: none; height: 132px;\" border=\"0\">\n<tbody>\n<tr style=\"height: 132px;\">\n<td style=\"width: 14.3925%; height: 132px;\"><a href=\"https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/05\/TECHNOTE_IQCA-RT50_1.0_EN.pdf\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/05\/IQCA-Cover-150x150.png\" width=\"150\" height=\"150\" alt=\"\" class=\"wp-image-4767 alignnone size-thumbnail\" \/><\/a><\/td>\n<td style=\"width: 85.4206%; height: 132px;\"><a href=\"https:\/\/entrogen.com\/web4\/wp-content\/uploads\/2025\/05\/TECHNOTE_IQCA-RT50_1.0_EN.pdf\" target=\"_blank\" rel=\"noopener\">Internal Quality Control Assay (IQCA) Application Note<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p>Click to download lot-specific quality control data for your product:<\/p>\n<p><u><a href=\"https:\/\/entrogen.com\/coa\"><a href=\"https:\/\/entrogen.com\/coa\" class=\"su-button su-button-style-default\" style=\"color:#FFFFFF;background-color:#2D89EF;border-color:#246ec0;border-radius:5px\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"color:#FFFFFF;padding:0px 16px;font-size:13px;line-height:26px;border-color:#6cadf4;border-radius:5px;text-shadow:none\"> Download<\/span><\/a><\/a><\/u><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n[\/et_pb_tab][\/et_pb_tabs][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_wc_related_products include_categories=&#8221;current&#8221; show_price=&#8221;off&#8221; show_rating=&#8221;off&#8221; show_sale_badge=&#8221;off&#8221; _builder_version=&#8221;4.27.2&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_wc_related_products][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_section]\n","protected":false},"excerpt":{"rendered":"<p>Cat Number: <strong>IQCA-RT50<\/strong><\/p>\n<p>The Internal Quality Control Assay is a qPCR-based test that verifies the presence of amplifiable human DNA in FFPE samples to ensure specimen integrity for downstream molecular analysis.<\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li>Assesses FFPE-derived DNA quality.<\/li>\n<li>Predicts optimal DNA input for maximal and reliable data output in EntroGen\u2019s qPCR assays.<\/li>\n<li>Saves samples, reagents, and time by eliminating the need for reruns.<\/li>\n<li>Simple set up and interpretation.<\/li>\n<li>Automated analysis available.<\/li>\n<li>For research use (RUO) in the U.S<\/li>\n<li>For in vitro diagnostic use (IVD) in the European Union<\/li>\n<\/ul>\n","protected":false},"featured_media":4927,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"[su_tabs][su_tab title=\"Overview\" disabled=\"no\" anchor=\"\" url=\"\" target=\"\" class=\"\"]\r\n\r\nMutations in the KRAS gene, commonly found in metastatic colorectal cancer (mCRC), lung adenocarcinoma, and thyroid cancer, can make tumors resistant to anti-EGFR treatments. Studies show that mutations in exons 2, 3, and 4 of the KRAS and NRAS genes reduce the effectiveness of these therapies, leading experts, including the American Society of Clinical Oncology (ASCO), to recommend genetic screening before treatment.\r\n\r\nThe RAS mutation analysis real-time assay utilizes allele-specific PCR, ensuring accurate detection using fluorescent hydrolysis probes. The panel is a qualitative test that detects somatic mutations found in exons 2, 3, and 4 of KRAS and NRAS genes. Below is a list of mutations detected by this kit.\r\n\r\nThe RAS Mutation Screening Panel identifies the presence of the mutations in a total of 8 reactions per sample. The test does not distinguish between mutations within each group.\r\n\r\n*A complementary add-on reaction that distinguishes KRAS G12C from other codon 12 mutations is available, upon request.\r\n\r\n<sup><img class=\"alignnone size-medium wp-image-4077\" src=\"http:\/\/entrogen.com\/web4\/wp-content\/uploads\/2024\/11\/KRAS-Mutation-Chart-300x128.png\" alt=\"\" width=\"300\" height=\"128\" \/>\r\n<\/sup><sup>1<\/sup> My Cancer Genome; www.mycancergenome.org (Colorectal Carcinoma, Colorectal Adenocarcinoma)\r\n\r\n[\/su_tab]\r\n[su_tab title=\"Workflow\" disabled=\"no\" anchor=\"\" url=\"\" target=\"blank\" class=\"\"]\r\n\r\nProduct Workflow\r\n\r\n[\/su_tab]\r\n[su_tab title=\"Resources\" disabled=\"no\" anchor=\"\" url=\"\" target=\"blank\" class=\"\"]\r\n\r\n&nbsp;\r\n\r\n&nbsp;\r\n\r\n<a href=\"http:\/\/entrogen.com\/web4\/videos\/ras.htm\">http:\/\/entrogen.com\/web4\/videos\/ras.htm<\/a>\r\n\r\n[\/su_tab][\/su_tabs]","_et_gb_content_width":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false},"product_brand":[],"product_cat":[39],"product_tag":[],"class_list":{"0":"post-4766","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-uncategorized","8":"first","9":"instock","10":"shipping-taxable","11":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product\/4766","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/media\/4927"}],"wp:attachment":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/media?parent=4766"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product_brand?post=4766"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product_cat?post=4766"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product_tag?post=4766"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}