This kit is for use with EntroGen\u2019s NGS assays only.\u00a0 May be purchased separately.\u00a0 It is also supplied with the following kits:<\/p>\n
- \n
- BRCA Complete (catalog no: BRCA-NGS)<\/a><\/li>\n<\/ul>\n
The reagents in the kit are sufficient for 75 triplicate reactions including all standards, controls, and samples.<\/p>\n
After preparing your libraries and diluting them to the recommended concentration, qPCR is performed using the provided 2X master mix, primer mix, and pre-diluted standards. The resulting Ct values are used to calculate the concentration of each library by comparison to the standard curve. The process enables accurate normalization of libraries for consistent cluster densities and optimal sequencing performance on Illumina\u00ae platforms.<\/p>\n
Reagents for DNA library preparation are not included.<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n
Click to download lot-specific quality control data for your product:<\/p>\n
Download<\/span><\/a><\/a><\/u><\/p>\n
<\/p>\n[\/et_pb_tab][\/et_pb_tabs][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_related_products include_categories=”current” show_price=”off” show_rating=”off” show_sale_badge=”off” _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_wc_related_products][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_section]\n","protected":false},"excerpt":{"rendered":"
Cat Number: LIBQ-NGS<\/strong><\/p>\n
The Library Quantification for Illumina\u00ae Kit is intended for the assessment of library quality for downstream Illumina\u00ae sequencing.<\/p>\n
<\/p>\n
- \n
- qPCR is the most accurate method for quantifying a sequencing library.<\/li>\n
- Qualifies and quantifies library molecules that can be sequenced in downstream Illumina assays.<\/li>\n
- Predicts library input for maximal and reliable data output.<\/li>\n
- Simple set up and interpretation.<\/li>\n
- Automated analysis available<\/li>\n
- For research use (RUO) in the U.S<\/li>\n
- For in vitro diagnostic use (IVD) in the European Union<\/li>\n<\/ul>\n","protected":false},"featured_media":4953,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"[su_tabs][su_tab title=\"Overview\" disabled=\"no\" anchor=\"\" url=\"\" target=\"\" class=\"\"]\r\n\r\nMutations in the KRAS gene, commonly found in metastatic colorectal cancer (mCRC), lung adenocarcinoma, and thyroid cancer, can make tumors resistant to anti-EGFR treatments. Studies show that mutations in exons 2, 3, and 4 of the KRAS and NRAS genes reduce the effectiveness of these therapies, leading experts, including the American Society of Clinical Oncology (ASCO), to recommend genetic screening before treatment.\r\n\r\nThe RAS mutation analysis real-time assay utilizes allele-specific PCR, ensuring accurate detection using fluorescent hydrolysis probes. The panel is a qualitative test that detects somatic mutations found in exons 2, 3, and 4 of KRAS and NRAS genes. Below is a list of mutations detected by this kit.\r\n\r\nThe RAS Mutation Screening Panel identifies the presence of the mutations in a total of 8 reactions per sample. The test does not distinguish between mutations within each group.\r\n\r\n*A complementary add-on reaction that distinguishes KRAS G12C from other codon 12 mutations is available, upon request.\r\n\r\n
![\"\"](\"http:\/\/entrogen.com\/web4\/wp-content\/uploads\/2024\/11\/KRAS-Mutation-Chart-300x128.png\")
\r\n<\/sup>1<\/sup> My Cancer Genome; www.mycancergenome.org (Colorectal Carcinoma, Colorectal Adenocarcinoma)\r\n\r\n[\/su_tab]\r\n[su_tab title=\"Workflow\" disabled=\"no\" anchor=\"\" url=\"\" target=\"blank\" class=\"\"]\r\n\r\nProduct Workflow\r\n\r\n[\/su_tab]\r\n[su_tab title=\"Resources\" disabled=\"no\" anchor=\"\" url=\"\" target=\"blank\" class=\"\"]\r\n\r\n \r\n\r\n \r\n\r\nhttp:\/\/entrogen.com\/web4\/videos\/ras.htm<\/a>\r\n\r\n[\/su_tab][\/su_tabs]","_et_gb_content_width":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false},"product_brand":[],"product_cat":[39],"product_tag":[],"class_list":{"0":"post-4773","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-uncategorized","8":"first","9":"instock","10":"shipping-taxable","11":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product\/4773","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/media\/4953"}],"wp:attachment":[{"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/media?parent=4773"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product_brand?post=4773"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product_cat?post=4773"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/entrogen.com\/web4\/wp-json\/wp\/v2\/product_tag?post=4773"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}