{"id":5266,"date":"2025-10-06T16:07:51","date_gmt":"2025-10-06T16:07:51","guid":{"rendered":"https:\/\/entrogen.com\/web4\/?post_type=product&p=5266"},"modified":"2025-11-10T19:21:20","modified_gmt":"2025-11-10T19:21:20","slug":"microsatellite-instability-kit","status":"publish","type":"product","link":"https:\/\/entrogen.com\/web4\/product\/microsatellite-instability-kit\/","title":{"rendered":"Microsatellite Instability Kit"},"content":{"rendered":"[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”6px||4px|||” collapsed=”off” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”100%” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_breadcrumb admin_label=”Woo Breadcrumbs” _builder_version=”4.16″ global_colors_info=”{}”][\/et_pb_wc_breadcrumb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”0px||9px|||” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_wc_images _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”|||-98px||” custom_padding=”|||23px||” global_colors_info=”{}”][\/et_pb_wc_images][\/et_pb_column][et_pb_column type=”2_3″ specialty_columns=”2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner column_structure=”1_2,1_2″ _builder_version=”4.27.2″ _module_preset=”default” custom_padding=”|0px||||” global_colors_info=”{}”][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_title _builder_version=”4.27.2″ _module_preset=”default” header_font_size=”27px” custom_margin=”|-164px||||” global_colors_info=”{}”][\/et_pb_wc_title][\/et_pb_column_inner][et_pb_column_inner type=”1_2″ saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column_inner][\/et_pb_row_inner][et_pb_row_inner _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”2_3″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_wc_description admin_label=”Woo Product Short Description” _builder_version=”4.27.4″ custom_margin=”-8px|||||” global_colors_info=”{}”][\/et_pb_wc_description][et_pb_wc_meta show_sku=”off” admin_label=”Woo Product Meta” _builder_version=”4.27.2″ global_colors_info=”{}”][\/et_pb_wc_meta][et_pb_button button_url=”https:\/\/entrogen.com\/web4\/product-information-request-form\/” button_text=”Request More Information” button_alignment=”left” admin_label=”Button” _builder_version=”4.27.4″ _module_preset=”default” custom_button=”on” button_text_size=”13px” button_text_color=”#FFFFFF” button_bg_color=”#476e8d” button_border_color=”RGBA(255,255,255,0)” background_layout=”dark” custom_margin=”30px|||||” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column_inner][\/et_pb_row_inner][\/et_pb_column][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” width=”100%” custom_padding=”6px|||||” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.27.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_tabs admin_label=”Tabs” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”24px|||||” custom_padding=”|30px|60px|||” global_colors_info=”{}”][et_pb_tab title=”Product Overview” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

Microsatellite instability (MSI) represents a key molecular signature of tumors with defective DNA mismatch repair (dMMR), a condition that leads to the accumulation of insertion or deletion errors within short, repetitive DNA sequences known as microsatellites. MSI is a hallmark of Lynch syndrome (hereditary nonpolyposis colorectal cancer) and is also commonly observed in several solid tumors, including colorectal, endometrial, and gastric cancers. Tumors exhibiting MSI-high (MSI-H) status have distinct biological and clinical characteristics, including increased neoantigen load and enhanced response to immune checkpoint inhibitors, making MSI testing an important tool in cancer research and therapeutic decision-making.<\/p>\n

The EntroGen MSI Detection Kit is a qPCR-based assay designed to detect and characterize MSI across five mononucleotide repeat regions: BAT-25 (KIT), BAT-26 (MSH2), NR-21 (SLC7A8), NR-27 (BIRC3), and MONO-27 (MAP4K3). The assay employs high-resolution melting (HRM) analysis to evaluate changes in DNA melting profiles caused by alterations in microsatellite length\u2014an indicator of MSI. By incorporating wildtype sequence blockers, the assay selectively suppresses normal sequence amplification, enhancing sensitivity for detecting MSI-induced shifts within tumor DNA.<\/p>\n

This five-reaction assay includes target-specific primer sets and MSI\/MSS controls for reliable baseline correction across HRM-enabled thermal cyclers. The method eliminates the need for capillary electrophoresis.<\/p>\n

The EntroGen MSI Detection Kit is compatible with a wide range of HRM-enabled real-time PCR instruments. To support streamlined and standardized interpretation, EntroGen provides dedicated analysis software that simplifies data processing and aids in accurate MSI status determination.<\/p>\n

\n\n\n\n\n\n\n\n\n
\n

Reaction No.<\/strong><\/p>\n<\/td>\n

\n

Reporter<\/strong><\/p>\n<\/td>\n

\n

Target<\/strong><\/p>\n<\/td>\n

\n

Gene<\/strong><\/p>\n<\/td>\n

\n

Chromosome<\/strong><\/p>\n<\/td>\n

\n

Position (hg38)<\/strong><\/p>\n<\/td>\n<\/tr>\n

\n

1<\/p>\n<\/td>\n

\n

FAM<\/p>\n<\/td>\n

\n

BAT-25<\/p>\n<\/td>\n

\n

KIT<\/p>\n<\/td>\n

\n

4<\/p>\n<\/td>\n

\n

54732045<\/p>\n<\/td>\n<\/tr>\n

\n

2<\/p>\n<\/td>\n

\n

BAT-26<\/p>\n<\/td>\n

\n

MSH2<\/p>\n<\/td>\n

\n

2<\/p>\n<\/td>\n

\n

47414420<\/p>\n<\/td>\n<\/tr>\n

\n

3<\/p>\n<\/td>\n

\n

NR-21<\/p>\n<\/td>\n

\n

SLC7A8<\/p>\n<\/td>\n

\n

14<\/p>\n<\/td>\n

\n

23183137<\/p>\n<\/td>\n<\/tr>\n

\n

4<\/p>\n<\/td>\n

\n

NR-27<\/p>\n<\/td>\n

\n

BIRC3<\/p>\n<\/td>\n

\n

11<\/p>\n<\/td>\n

\n

102322777<\/p>\n<\/td>\n<\/tr>\n

\n

5<\/p>\n<\/td>\n

\n

MONO-27<\/p>\n<\/td>\n

\n

MAP4K3<\/p>\n<\/td>\n

\n

2<\/p>\n<\/td>\n

\n

39345921<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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The MSI Detection Kit follows a simple, streamlined process that can be completed in approximately 3 hours, from DNA preparation to result generation.<\/p>\n

EntroGen\u02bcs MSI Detection Kit requires a real-time PCR instrument capable of detecting FAM fluorescent probes.\u00a0<\/p>\n

This test includes reagents required for PCR amplification\/detection, as well as validated reaction controls.\u00a0A single 96-well plate can accommodate up to six unknown samples in duplicate along with the required controls.\u00a0<\/p>\n

Columns and reagents for DNA isolation are not included. Genomic DNA from FFPE tissues can be extracted using EntroGen pureNA\u00ae Genomic DNA Isolation Kit<\/a>.<\/p>\n

To support data interpretation, EntroGen provides dedicated MSI Analysis Software that simplifies melting curve analysis and aids in accurate determination of MSI status.<\/p>\n

\"\"\u00a0\"\"\u00a0\u00a0\"\"\u00a0\"\" \u00a0\u00a0\u00a0\"\"<\/p>\n[\/et_pb_tab][et_pb_tab title=”Resources” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]\n

Click to download lot-specific quality control data for your product:<\/p>\n

Download<\/span><\/a><\/a><\/u><\/p>\n

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Cat Number: MSI-RT24<\/strong><\/p>\n

The MSI Kit is a qPCR-based assay that detects microsatellite instability (MSI) in five mononucleotide repeat regions using high-resolution melting (HRM) analysis to enable rapid, sensitive, and streamlined MSI assessment in colorectal tumor samples.<\/p>\n

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