BRCA Complete™

BRCA Complete™ Expanded Panel is a complete next generation sequencing (NGS) solution for detecting clinically relevant BRCA1 and BRCA2 mutations along with extended coverage of CHEK2, PALB2, RAD51C and TP53.

EntroGen’s BRCA Complete™ Expanded Panel is a complete NGS solution from DNA to interpreting sequencing data that detects germline and somatic mutations with comprehensive coverage, specificity and high sensitivity.  In addition to BRCA1 and BRCA2, the enhanced BRCA Complete™ Expanded Panel now covers CHEK2, PALB2, RAD51C and TP53.

BRCA Complete™ Expanded Panel is compatible with blood, fresh frozen, and formalin fixed paraffin embedded (FFPE) samples. Allele dropouts and off-target reads are reduced through a tiled amplicon PCR approach coupled with a high fidelity polymerase for target enrichment. Reagents for library preparation and clean-up are included, as well as local, user-friendly software designed to reduce the bioinformatics burden on the end-user by calling clinically relevant mutations directly from VCF files. Sample and library quality control assays (DNA Fragmentation Quantification assay, Library Quantification Kit for Illumina®) are available to ensure high quality data without the need for repeat sequencing.

From DNA to results in approximately 24 hours with 4 hours of hands-on time including sample and library QC.

BRCA Complete™ Expanded Panel offers all reagents necessary for target enrichment and library construction for Illumina® platforms from DNA isolated from patient samples.  Sample quality assessment assays (DNA Fragmentation Quantification assay, Library Quantification Kit for Illumina®) are available to ensure samples and sequencing reagents are not wasted. The complimentary software queries two independent databases to call clinically relevant variants based on Illumina® output reports.

EntroGen Variant Analysis (EVA)

Data Analysis Software

BRCA Complete™ Expanded Panel comes with user-friendly, locally installed software to streamline reporting and simplify data interpretation.  VCF sequencing files are queried by two independent databases providing end-users with the latest, most clinically relevant biomedical information for each detected variant.  Our automated reporting software is designed to reduce the amount of time, cost and complexity associated with data analysis by minimizing the time spent interpreting data.  Comprehensive reports allow end-users to quickly determine variant calling, clinical relevance, treatment recommendations and current clinical trials.  Reports can also be easily exported and filtered for a more focused data review.

Equipment & Materials:

BRCA Complete™ Expanded Panel require the following equipment:

• Illumina® MiniSeq. Illumina® MiSeq or NextSeq 500 are also compatible.
• PCR thermocycler.
• Real-time PCR machine (for sample and library QC assays).
• Magnetic rack (for amplicon and library clean-up).

The panel includes reagents for:

• Target enrichment.
• Library preparation (indexes and adaptor oligos for Illumina® sequencing).
• Amplicon and library clean-up (magnetic beads are included).

The reagents in the kits are to prepare sequencing libraries for up to 40 samples.