NRAS Mutation Detection Kit

Available Products

Product Name/Description
No. of Reactions*
Product Code
NRAS Mutation Detection Kit
50
NRAS-RT50
RAS c.59/117 Mutation Detection Kit †
50
RASX-RT50

* Includes all controls. † For detection of KRAS c.59, NRAS c.59/117 somatic mutations.  Available with purchase of NRAS-RT50, KRAS-RT50, or KRBR-RT50.

NRAS Mutations and Cancer

NRAS is a member of the RAS family of GTPases and plays a central role in the MAPK signaling pathway.  Activating mutations in exon 2 (codon 12/13), exon 3 (codon 61), and exon 4 (codon 146) have been found in various cancers, including melanoma (13-25%), colorectal cancer (1-6%), lung cancer (1%), hepatocellular carcinoma (10%), myeloid leukemias (14%), and thyroid carcinoma (7%). The table below lists the mutations detected by the NRAS Mutation Detection kit.

Exon Mutation Name Nucleotide Change Amino Acid Change
2 G12C c.34G>T Glycine (G) to Cystein (C)
G12D c.35G>A Glycine (G) to Aspartic acid (D)
G12S c.34G>A Glycine (G) to Serine (S)
G13V c.38G>T Glycine (G) to Valine (V)
G13R c.37G>C Glycine (G) to Arginine (R)
3 Q61K c.181C>A Glutamine (Q) to Lysine (K)
Q61R c.182A>G Glutamine (Q) to Arginine (R)
Q61L c.182A>T Glutamine (Q) to Leucine (L)
Q61H c.183A>C & c.183A>T Glutamine (Q) to Histidine (H)
4 A146T c.436G>A Alanine (A) to Threonine (T)

For expanded coverage, the RAS c.59/117 Mutation Detection Kit detects KRAS c.59, NRAS c.59/117 somatic mutations.   EntroGen’s NRAS mutation detection kit is a polymerase chain reaction (PCR)-based assay that uses allele-specific primers to identify the presence of the mutations listed above. The testing procedure involves three (3) simple steps:

  • Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), or fresh frozen tumors
  • Amplification of regions of the NRAS gene using allele-specific primers.
  • Detection of amplification product on a Real-Time PCR instrument.

This test can be completed in approximately 2 hours from isolation of DNA to test result.

Equipment and Materials

EntroGen’s NRAS mutation detection kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. This test includes reagents required for PCR amplification/detection, as well as validated reaction controls. Columns and reagents for DNA isolation are not included.

Intended Use

USA: EntroGen’s NRAS mutation analysis kit is provided for research use only (RUO). Not for use in diagnostic procedures. Europe: EntroGen’s NRAS mutation analysis kit is available for research (RUO) and diagnostic (CE-IVD) purposes.

Bibliography of studies using this product

Cetuximab in Treatment of Metastatic Colorectal Cancer: Final Survival Analyses and Extended RAS Data from the NORDIC-VII Study. Guren, Thomsen, Kure, Sorbye, Glimelius, Pfeiffer, Österlund, Sigurdsson, Lothe, Dalsgaard, Skovlund, Christoffersen, Tveit. Comparison of KRAS Mutation Tests in Colorectal Cancer Patients. Br J Cancer. 2017 May 9;116(10):1271-1278. Pubmed ID 28399112

Analysis of KRAS, NRAS, BRAF, and PIK3CA Mutations Could Predict Metastases in Colorectal Cancer: A Preliminary Study. Wojas-Krawczyk K, Kalinka-Warzocha E, Reszka K, Nicoś M, Szumiło J, Mańdziuk S, Szczepaniak K, Kupnicka D, Lewandowski R, Milanowski J, Krawczyk P. Adv Clin Exp Med. 2018 Aug 7. Pubmed ID 30085422

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