NRAS Mutation Detection Kit
No. of Reactions*
|NRAS Mutation Analysis Kit||
|RAS c.59/117 Mutation Detection Kit †||
* Includes all controls. † For detection of KRAS c.59, NRAS c.59/117 somatic mutations. Available with purchase of NRAS-RT50, KRAS-RT50, or KRBR-RT50.
NRAS Mutations and Cancer
NRAS is a member of the RAS family of GTPases and plays a central role in the MAPK signaling pathway. Activating mutations in exon 2 (codon 12/13), exon 3 (codon 61), and exon 4 (codon 146) have been found in various cancers, including melanoma (13-25%), colorectal cancer (1-6%), lung cancer (1%), hepatocellular carcinoma (10%), myeloid leukemias (14%), and thyroid carcinoma (7%). The table below lists the mutations detected by the NRAS Mutation Detection kit.
|Exon||Mutation Name||Nucleotide Change||Amino Acid Change|
|2||G12C||c.34G>T||Glycine (G) to Cystein (C)|
|G12D||c.35G>A||Glycine (G) to Aspartic acid (D)|
|G12S||c.34G>A||Glycine (G) to Serine (S)|
|G13V||c.38G>T||Glycine (G) to Valine (V)|
|G13R||c.37G>C||Glycine (G) to Arginine (R)|
|3||Q61K||c.181C>A||Glutamine (Q) to Lysine (K)|
|Q61R||c.182A>G||Glutamine (Q) to Arginine (R)|
|Q61L||c.182A>T||Glutamine (Q) to Leucine (L)|
|Q61H||c.183A>C & c.183A>T||Glutamine (Q) to Histidine (H)|
|4||A146T||c.436G>A||Alanine (A) to Threonine (T)|
For expanded coverage, the RAS c.59/117 Mutation Detection Kit detects KRAS c.59, NRAS c.59/117 somatic mutations. EntroGen’s NRAS mutation detection kit is a polymerase chain reaction (PCR)-based assay that uses allele-specific primers to identify the presence of the mutations listed above. The testing procedure involves three (3) simple steps:
- Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), or fresh frozen tumors
- Amplification of regions of the NRAS gene using allele-specific primers.
- Detection of amplification product on a Real-Time PCR instrument.
This test can be completed in approximately 2 hours from isolation of DNA to test result.
Equipment and Materials
EntroGen’s NRAS mutation detection kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. This test includes reagents required for PCR amplification/detection, as well as validated reaction controls. Columns and reagents for DNA isolation are not included.
USA: EntroGen’s NRAS mutation analysis kit is provided for research use only (RUO). Not for use in diagnostic procedures. Europe: EntroGen’s NRAS mutation analysis kit is available for research (RUO) and diagnostic (CE-IVD) purposes.
Bibliography of studies using this product
Cetuximab in Treatment of Metastatic Colorectal Cancer: Final Survival Analyses and Extended RAS Data from the NORDIC-VII Study. Guren, Thomsen, Kure, Sorbye, Glimelius, Pfeiffer, Österlund, Sigurdsson, Lothe, Dalsgaard, Skovlund, Christoffersen, Tveit. Comparison of KRAS Mutation Tests in Colorectal Cancer Patients. Br J Cancer. 2017 May 9;116(10):1271-1278. Pubmed ID 28399112
Analysis of KRAS, NRAS, BRAF, and PIK3CA Mutations Could Predict Metastases in Colorectal Cancer: A Preliminary Study. Wojas-Krawczyk K, Kalinka-Warzocha E, Reszka K, Nicoś M, Szumiło J, Mańdziuk S, Szczepaniak K, Kupnicka D, Lewandowski R, Milanowski J, Krawczyk P. Adv Clin Exp Med. 2018 Aug 7. Pubmed ID 30085422